To explore the application and effect of optometry assessment based on mini clinical evaluation exercise (Mini-CEX) scale and direct observation of procedural skills (DOPS) scale in comprehensive training, students of 2020 Optometry Major were selected as the control group and the observation group. The control group adopted the traditional teaching method. While the observation group adopted optometryassessment based on mini-CEX and DOPS, using assessment-feedback-improvement-reassessment teaching method. The test scores of students in both groups after the comprehensive training were higher than those before the comprehensive training, and the test scores of the observation group students were higher than those of the control group students (P<0.001).The students in the observation group were more satisfied with the teaching method of comprehensive training and thought that the comprehensive training was conducive to faster and better adaptation to the practice than those in the control group (P<0.05). The three-time assessment scores of the students in the observation group increased successively, and the difference was statistically significant (P<0.001). The optometry assessment based on mini-CEX and DOPS can improve the teaching effect of comprehensive training significantly and make students adapt to the internship position faster.