Acta Optica Sinica, Volume. 44, Issue 14, 1426003(2024)

Customization of Broadband Speckle and Its Application in Single-Shot Multi-Color Fluorescence Super-Resolution Microscopic Ghost Imaging

Li Chen1,2,3,4, Xiaohan Wan1,2,3,4, Pengwei Wang1,3,4,5, Zhentao Liu1,2,3,4、*, Jianrong Wu1,3,4, and Shensheng Han1,2,3,4,6
Author Affiliations
  • 1Key Laboratory of Quantum Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
  • 2Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
  • 3Wangzhijiang Innovation Center for Laser, Aerospace Laser Technology and System Department, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
  • 4Key Laboratory of Space Laser Communication and Detection Technology, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
  • 5Key Laboratory of Advanced Optical Manufacturing Technologies of Jiangsu Province, Soochow University, Suzhou 215006, Jiangsu , China
  • 6Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, Zhejiang , China
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    Figures & Tables(9)
    Flow chart of designing the customization of broadband speckle modulation
    Customizing speckles with different statistical distribution in the wavelength range of 500-525 nm. (a1) Sub-Rayleigh and (a2) super-Rayleigh speckle patterns at modulated wavelengths; change in (b1) sub-Rayleigh and (b2) super-Rayleigh speckle contrast with wavelength
    Customizing super-Rayleigh speckles with different modulated wavelength numbers in the wavelength range of 500-700 nm. (a)-(c) Speckle patterns at different wavelengths when the modulation wavelength number is 1, 5, and 21; (d) changes in speckle contrast with wavelength under different modulation wavelength numbers
    Customizing multi-wavelength super-Rayleigh speckles in simulation and experiment. (a) Simulated super-Rayleigh speckle patterns at modulated wavelengths; (b) super-Rayleigh speckle patterns at modulated wavelengths generated by a phase modulator in experiment; (c) change in speckle contrast with wavelength
    Schematic of single-shot multi-color fluorscence super-resolution microscopy ghost imaging system
    Simulation results of multi-color imaging. (a1)-(a3) Ground truth of fluorescent microspheres with wavelengths of 521, 603, and 690 nm, respectively; (a4) three-color target (red, green, and blue represent 521, 603, and 690 nm fluorescent microspheres); (b1)-(b4) reconstructed results of three-color fluorescent microspheres using multi-wavelengths super-Rayleigh speckle modulation when the detection signal-to-noise ratio is 8.32 dB
    Comparison of simulation reconstruction results between Rayleigh and super-Rayleigh speckle modulation under different photon numbers. (a)(b) Reconstructed results by Rayleigh and super-Rayleigh speckle modulation under different photons numbers; (c)(d) SSIM and PSNR value of the reconstructed three-color object using two speckle modulation ways under different photons numbers
    • Table 1. Effect of fluorescent microspheres size on reconstruction results

      View table

      Table 1. Effect of fluorescent microspheres size on reconstruction results

      Np2 pixel×2 pixel4 pixel×4 pixel6 pixel×6 pixel
      SSIMPSNR /dBSSIMPSNR /dBSSIMPSNR /dB
      50.799722.93350.763619.90000.618116.5399
      200.878124.87020.869723.52250.766821.3820
      1000.948628.65900.936127.82350.890825.6907
      10000.981932.99700.964930.88890.944328.2414
      100000.987535.66050.975231.99370.963329.3170
    • Table 2. Effect of fluorescent microspheres density on reconstruction results

      View table

      Table 2. Effect of fluorescent microspheres density on reconstruction results

      Npρ=0.083 µm-2ρ=0.277 µm-2ρ=0.555 µm-2
      SSIMPSNR /dBSSIMPSNR /dBSSIMPSNR /dB
      50.946328.36630.799722.93350.663519.7768
      200.975931.79780.878124.87020.764721.5911
      1000.988635.15690.948628.65900.902725.3550
      10000.992937.85420.981932.99700.967129.8225
      100000.995839.37690.987535.66050.975931.9777
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    Li Chen, Xiaohan Wan, Pengwei Wang, Zhentao Liu, Jianrong Wu, Shensheng Han. Customization of Broadband Speckle and Its Application in Single-Shot Multi-Color Fluorescence Super-Resolution Microscopic Ghost Imaging[J]. Acta Optica Sinica, 2024, 44(14): 1426003

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    Paper Information

    Category: Physical Optics

    Received: Mar. 11, 2024

    Accepted: Apr. 16, 2024

    Published Online: Jul. 17, 2024

    The Author Email: Liu Zhentao (ztliu@siom.ac.cn)

    DOI:10.3788/AOS240717

    CSTR:32393.14.AOS240717

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