Acta Optica Sinica, Volume. 44, Issue 14, 1426003(2024)
Customization of Broadband Speckle and Its Application in Single-Shot Multi-Color Fluorescence Super-Resolution Microscopic Ghost Imaging
Fig. 1. Flow chart of designing the customization of broadband speckle modulation
Fig. 2. Customizing speckles with different statistical distribution in the wavelength range of 500-525 nm. (a1) Sub-Rayleigh and (a2) super-Rayleigh speckle patterns at modulated wavelengths; change in (b1) sub-Rayleigh and (b2) super-Rayleigh speckle contrast with wavelength
Fig. 3. Customizing super-Rayleigh speckles with different modulated wavelength numbers in the wavelength range of 500-700 nm. (a)-(c) Speckle patterns at different wavelengths when the modulation wavelength number is 1, 5, and 21; (d) changes in speckle contrast with wavelength under different modulation wavelength numbers
Fig. 4. Customizing multi-wavelength super-Rayleigh speckles in simulation and experiment. (a) Simulated super-Rayleigh speckle patterns at modulated wavelengths; (b) super-Rayleigh speckle patterns at modulated wavelengths generated by a phase modulator in experiment; (c) change in speckle contrast with wavelength
Fig. 5. Schematic of single-shot multi-color fluorscence super-resolution microscopy ghost imaging system
Fig. 6. Simulation results of multi-color imaging. (a1)-(a3) Ground truth of fluorescent microspheres with wavelengths of 521, 603, and 690 nm, respectively; (a4) three-color target (red, green, and blue represent 521, 603, and 690 nm fluorescent microspheres); (b1)-(b4) reconstructed results of three-color fluorescent microspheres using multi-wavelengths super-Rayleigh speckle modulation when the detection signal-to-noise ratio is 8.32 dB
Fig. 7. Comparison of simulation reconstruction results between Rayleigh and super-Rayleigh speckle modulation under different photon numbers. (a)(b) Reconstructed results by Rayleigh and super-Rayleigh speckle modulation under different photons numbers; (c)(d) SSIM and PSNR value of the reconstructed three-color object using two speckle modulation ways under different photons numbers
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Li Chen, Xiaohan Wan, Pengwei Wang, Zhentao Liu, Jianrong Wu, Shensheng Han. Customization of Broadband Speckle and Its Application in Single-Shot Multi-Color Fluorescence Super-Resolution Microscopic Ghost Imaging[J]. Acta Optica Sinica, 2024, 44(14): 1426003
Category: Physical Optics
Received: Mar. 11, 2024
Accepted: Apr. 16, 2024
Published Online: Jul. 17, 2024
The Author Email: Liu Zhentao (ztliu@siom.ac.cn)
CSTR:32393.14.AOS240717