Journal of Jiangsu University(Medicine Edition), Volume. 35, Issue 4, 304(2025)

The inhibitory effect and mechanism of neural stem cell secretome on ferroptosis in human neuroblastoma SH-SY5Y cells

ZENG Yu1, QIU Yun1, SI Yu1, GUO Tao1, NI Yinying1, ZHANG Jingwen1, WANG Ziyu2, CHEN Bo3, and HU Jiabo1,3、*
Author Affiliations
  • 1School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013
  • 2Health Clinical Laboratories, Health BioMed Co., Ltd.Ningbo Zhejiang 315042
  • 3Institute of Cerebrovascular Disease, Jiangsu University, Zhenjiang Jiangsu 212002, China
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    References(25)

    [1] [1] Stockwell BR, Friedmann Angeli JP, Bayir H, et al. Ferroptosis: A regulated cell death nexus linking metabolism, redox biology, and disease[J]. Cell, 2017, 171(2): 273-285.

    [2] [2] Lee H, Horbath A, Kondiparthi L, et al. Cell cycle arrest induces lipid droplet formation and confers ferroptosis resistance[J]. Nat Commun, 2024, 15(1): 79.

    [3] [3] Zhang W, Liu Y, Liao Y, et al. GPX4, ferroptosis, and diseases[J]. Biomed Pharmacother, 2024, 174: 116512.

    [4] [4] Liu W, Chakraborty B, Safi R, et al. Dysregulated cholesterol homeostasis results in resistance to ferroptosis increasing tumorigenicity and metastasis in cancer[J]. Nat Commun, 2021, 12(1): 5103.

    [5] [5] Fu Y, Zhou X, Wang L, et al. Salvianolic acid B attenuates liver fibrosis by targeting Ecm1 and inhibiting hepatocyte ferroptosis[J]. Redox Biol, 2024, 69: 103029.

    [6] [6] Deng Y, Zeng L, Liu H, et al. Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1[J]. Redox Biol, 2024, 77: 103360.

    [7] [7] Wang H, Mao W, Zhang Y, et al. NOX1 triggers ferroptosis and ferritinophagy, contributes to Parkinson′s disease[J]. Free Radic Biol Med, 2024, 222: 331-343.

    [8] [8] Wei C, Xiao Z, Zhang Y, et al. Itaconate protects ferroptotic neurons by alkylating GPx4 post stroke[J]. Cell Death Differ, 2024, 31(8): 983-998.

    [9] [9] Liu D, Yang S, Yu S. Interactions between ferroptosis and oxidative stress in ischemic stroke[J]. Antioxidants(Basel), 2024, 13(11): 1329.

    [10] [10] Zhi SM, Cui Y, Liu Y, et al. Paeoniflorin suppresses ferroptosis after traumatic brain injury by antagonizing P53 acetylation[J]. Phytomedicine, 2024, 133: 155940.

    [11] [11] Ni W, Zhou J, Ling Y, et al. Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson′s disease model[J]. Brain Res, 2022, 1790: 147978.

    [12] [12] Lv X, Ling Y, Niu D, et al. Human neural stem cell secretome inhibits neuron heme uptake and ferroptosis in intracerebral hemorrhage through Nrf-2 signaling pathway[J]. Stem Cells Dev, 2023, 32(11/12): 346-363.

    [14] [14] Chen X, Ye K, Yu J, et al. Regeneration of sciatic nerves by transplanted microvesicles of human neural stem cells derived from embryonic stem cells[J]. Cell Tissue Bank, 2020, 21(2): 233-248.

    [15] [15] Zhou J, Ni W, Ling Y, et al. Human neural stem cell secretome inhibits lipopolysaccharide-induced neuroin-flammation through modulating microglia polarization by activating peroxisome proliferator-activated receptor gamma[J]. Stem Cells Dev, 2022, 31(13/14): 369-382.

    [16] [16] Li Y, Zeng X, Lu D, etal. Erastininduces ferroptosis via ferroportin-mediated iron accumulation in endometriosis[J]. Hum Reprod, 2021, 36(4): 951-964.

    [17] [17] Ling Y, Ramalingam M, Lv X, et al. Human neural stem cell secretome relieves endoplasmic reticulum stress-induced apoptosis and improves neuronal functions after traumatic brain injury in a rat model[J]. J Mol Histol, 2024, 55(3): 329-348.

    [18] [18] Dixon SJ, Olzmann JA. The cell biology of ferroptosis[J]. Nat Rev Mol Cell Biol, 2024, 25(6): 424-442.

    [19] [19] Rodriguez R, Schreiber SL, Conrad M. Persister cancer cells: Iron addiction and vulnerability to ferroptosis[J]. Mol Cell, 2022, 82(4): 728-740.

    [20] [20] Liu J, Tang D, Kang R. Targeting GPX4 in ferroptosis and cancer: chemical strategies and challenges[J]. Trends Pharmacol Sci, 2024, 45(8): 666-670.

    [21] [21] Pfister K, Young V, Frankel B, et al. Succinylation of Park7 activates a protective metabolic response to acute kidney injury[J]. Am J Physiol Renal Physiol, 2024, 327(1): F128-F136.

    [22] [22] Cai J, Kong D, Long Z, et al. Targeting PARK7 improves acetaminophen-induced acute liver injury by orchestrating mitochondrial quality control and metabolic reprogramming[J]. Antioxidants(Basel), 2022, 11(11): 2128.

    [23] [23] Lind-Holm Mogensen F, Scafidi A, Poli A, et al. PARK7/DJ-1 in microglia: implications in Parkinson′s disease and relevance as a therapeutic target[J]. J Neuroinflammation, 2023, 20(1): 95.

    [24] [24] Zhao L, Yang H, Wang Y, et al. STUB1 suppresses paclitaxel resistance in ovarian cancer through mediating HOXB3 ubiquitination to inhibit PARK7 expression[J]. Commun Biol, 2024, 7(1): 1439.

    [25] [25] Zhou J, Qi C, Fang X, et al. DJ-1 modulates Nrf2-mediated MRP1 expression by activating Wnt3a/-catenin signalling in A549 cells exposed to cigarette smoke extract and LPS[J]. Life Sci, 2021, 276: 119089.

    [26] [26] Anandhan A, Dodson M, Shakya A, et al. NRF2 controls iron homeostasis and ferroptosis through HERC2 and VAMP8[J]. Sci Adv, 9(5): eade9585.

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    ZENG Yu, QIU Yun, SI Yu, GUO Tao, NI Yinying, ZHANG Jingwen, WANG Ziyu, CHEN Bo, HU Jiabo. The inhibitory effect and mechanism of neural stem cell secretome on ferroptosis in human neuroblastoma SH-SY5Y cells[J]. Journal of Jiangsu University(Medicine Edition), 2025, 35(4): 304

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    Paper Information

    Special Issue:

    Received: Mar. 5, 2024

    Accepted: Aug. 21, 2025

    Published Online: Aug. 21, 2025

    The Author Email: HU Jiabo (hu@ujs.edu.cn)

    DOI:10.13312/j.issn.1671-7783.y240027

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