Objective:To explore the effect of neural stem cell secretome(NSC-S)on ferroptosis human neuroblastoma SH-SY5Y cells and its underlying mechanism.Methods:Human neuroblastoma SH-SY5Y cells were used to establish neuronal ferroptosis model in vitro. And the cells were divided into three groups: control group, Erastin group and Erastin+NSC-S group; cells were treated with DMEM, DMEM containing 10 μmol/L Erastin, and NSC-S containing 10 μmol/L Erastin, respectively. The activities of lactate dehydrogenase(LDH), intracellular reactive oxygen species and malondialdehyde in the supernatants of cells in each group were detected by commercial kits, and the contents of free ferrous ions were detected by fluorescent probes. The expression level of glutathione peroxidase 4(GPX4)protein in cells was determined by Western blotting. The components of NSC-S were detected by liquid chromatography tandem mass spectrometry(LC-MS/MS)and bioinformatics analysis and screening were conducted. In addition, SH-SY5Y cells were divided into four groups, which were control group, Erastin group, Erastin+NSC-S group, and Erastin+Parkinson′s disease protein 7(PARK7)group. And cells were treated with DMEM, DMEM containing 10 μmol/L Erastin, NSC-S containing 10 μmol/L Erastin, and DMEM containing 200 μg/L PARK7+10 μmol/L Erastin, respectively. The viability of SH-SY5Y cells was detected by the CCK-8 assay, and the contents of reactive oxygen species, malondialdehyde, ferrous ions in the cells as well as the expression level of GPX4 protein were also detected.Results:Compared with the Erastin group, the LDH activity of SH-SY5Y cells supernatant in the Erastin+NSC-S group was significantly decreased(P<0.001), the contents of intracellular reactive oxygen species, malondialdehyde and ferrous ions were significantly reduced(P<0.001 or P<0.05), and the relative expression level of GPX4 protein was greatly increased(P<0.05). LC-MS/MS analysis revealed that NSC-S contained PARK7 protein. Compared with the Erastin group, the viability of SH-SY5Y cells in the Erastin+PARK7 group was significantly enhanced(P<0.001), the contents of reactive oxygen species, malondialdehyde and ferrous ions were significantly decreased(P<0.001), and the relative expression level of GPX4 protein was significantly increased(P<0.05).Conclusion:NSC-S could inhibit ferroptosis induced by Erastin in human neuroblastoma SH-SY5Y cells, which may be related to the mediation of PARK7 in NSC-S.