Acta Physica Sinica, Volume. 69, Issue 8, 088701-1(2020)

High-speed and large-scaled light-sheet microscopy with electrically tunable lens

Yu-Yao Hu1,2, Dong Liang1,2, Jing Wang1, and Jun Liu1、*
Author Affiliations
  • 1State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
  • show less
    Figures & Tables(11)
    Schematic diagram of the cylindrical lens.
    Schematic diagram of rapid light-sheet fluorescence microscopy imaging system.
    Typical optical response of the EL-10-30-Ci to a current step.
    The beam passing through the ETL at different aperture angles: (a) The beam passing through the ETL at small aperture angles; (b) the beam passing through the ETL at big aperture angles.
    The beam passing through the ETL: (a) The beam is blocked by the aperture of ETL; (b) the beam is unblocked by the aperture of ETL.
    Analysis of 100 nm fluorescent spheres: (a) Image of 100 nm fluorescent spheres; (b)−(f) five fluorescent spheres with the largest intensity; (g)−(k) intensity profile of a horizontal line passing through the center of fluorescent sphere in (b)−(f); (l) the normalized and averaged curves of (g)−(k).
    Gaussian fitting curve.
    Fluorescence intensity distribution at different profile of the light sheet: (a) Optical path for recording light sheet; (b) profile of light sheet; (c) average curves of intensity profile of light sheet.
    Image of the same fluorescent sphere at different positions of the light sheet.
    Images of a 200 μm grid over the ETL scan range: (a) Image when IETL = 0 mA; (b) image when IETL = 146 mA; (c) image when IETL = 292 mA.
    The artifacts analysis of the experimental results: (a) Intensity profile of a line passing through the center of fluorescent sphere captured at 0 ms; (b) intensity profile of a line passing through the center of fluorescent sphere captured at 3.7 ms; (c) intensity profile of a line passing through the center of fluorescent sphere captured at 7.4 ms.
    Tools

    Get Citation

    Copy Citation Text

    Yu-Yao Hu, Dong Liang, Jing Wang, Jun Liu. High-speed and large-scaled light-sheet microscopy with electrically tunable lens[J]. Acta Physica Sinica, 2020, 69(8): 088701-1

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category:

    Received: Dec. 17, 2019

    Accepted: --

    Published Online: Nov. 24, 2020

    The Author Email:

    DOI:10.7498/aps.69.20191908

    Topics