Acta Physica Sinica, Volume. 69, Issue 8, 088701-1(2020)
Fig. 2. Schematic diagram of rapid light-sheet fluorescence microscopy imaging system.
Fig. 3. Typical optical response of the EL-10-30-Ci to a current step.
Fig. 4. The beam passing through the ETL at different aperture angles: (a) The beam passing through the ETL at small aperture angles; (b) the beam passing through the ETL at big aperture angles.
Fig. 5. The beam passing through the ETL: (a) The beam is blocked by the aperture of ETL; (b) the beam is unblocked by the aperture of ETL.
Fig. 6. Analysis of 100 nm fluorescent spheres: (a) Image of 100 nm fluorescent spheres; (b)−(f) five fluorescent spheres with the largest intensity; (g)−(k) intensity profile of a horizontal line passing through the center of fluorescent sphere in (b)−(f); (l) the normalized and averaged curves of (g)−(k).
Fig. 8. Fluorescence intensity distribution at different profile of the light sheet: (a) Optical path for recording light sheet; (b) profile of light sheet; (c) average curves of intensity profile of light sheet.
Fig. 9. Image of the same fluorescent sphere at different positions of the light sheet.
Fig. 10. Images of a 200 μm grid over the ETL scan range: (a) Image when
Fig. 11. The artifacts analysis of the experimental results: (a) Intensity profile of a line passing through the center of fluorescent sphere captured at 0 ms; (b) intensity profile of a line passing through the center of fluorescent sphere captured at 3.7 ms; (c) intensity profile of a line passing through the center of fluorescent sphere captured at 7.4 ms.
Get Citation
Copy Citation Text
Yu-Yao Hu, Dong Liang, Jing Wang, Jun Liu.
Category:
Received: Dec. 17, 2019
Accepted: --
Published Online: Nov. 24, 2020
The Author Email: