The Silica microsphere lens with low refractive index and BaTiO3 microsphere lenses with high refractive index are used to image the sub-wavelength surface structure of data-recorded Blu-ray disc. The imaging properties of the microsphere lenses when they are spatially separated from the surface of the sample are observed in the experiments. The experimental results illustrate that the sub-wavelength surface patterns can still be resolved by the microsphere lens with a significant magnification, when the distance between the microsphere lens and the sample surface is about 0~6μm. The comparative experiment shows that the high-index microsphere perform different imaging properties in immersing mode, magnification and imaging scale, et al. The simulation results of electric filed distribution of the microsphere lenses illuminate that the imaging properties of microspher lenses are greatly effected by the electric filed distribution, which provide basic for the revealing of the mechanism of super resolution of microsphere lenses.