Femtosecond lasers with different parameters (wavelength, pulse energy, pulse number) are used to process micro-holes of carbon nanotubes film. A theoretical calculation of the single-pulse ablation threshold of carbon nanotubes film for different wavelengths is made by fitting experimental results. The calculation results are 25 mJ·cm -2 at a wavelength of 1030 nm and 39.7 mJ·cm -2 at a wavelength of 515 nm. The influences of laser parameters on material processing results are investigated. The results indicate that the pulse energy of the laser is the primary factor influencing the diameter of the ablation, and a larger area of carbon nanotubes thrown area can be produced by a short wavelength femtosecond laser. Raman spectroscopy is used to assess the incisions of materials cut by femtosecond lasers with different wavelengths, and the results show that a femtosecond laser with a wavelength of 515 nm is more suitable for cutting carbon nanotubes film. The influences of pulse energy and scanning speed on the cutting quality are analyzed. The desired cutting quality is obtained under the optimized process parameters.