Pathological Analysis of Cutaneous Squamous Cell Carcinoma Based on Multispectral Microscopic Imaging

Cheng Wang; Changxing Yang; Jiayi Yang; Qianqian Ge; Wenqiang Cai; Yuling Yan; Huazhong Xiang; Dawei Zhang; Xiaoqing Zhao

doi:10.3788/CJL240584

Chinese Journal of Lasers, Volume. 51, Issue 15, 1507204(2024)

Pathological Analysis of Cutaneous Squamous Cell Carcinoma Based on Multispectral Microscopic Imaging

Cheng Wang^1,2、*, Changxing Yang^1,2, Jiayi Yang³, Qianqian Ge^1,2, Wenqiang Cai^1,2, Yuling Yan^1,2, Huazhong Xiang^1,2, Dawei Zhang⁴, and Xiaoqing Zhao³

Author Affiliations

¹School of Health Sciences and Engineering, Institute of Biomedical Optics and Optometry, University of Shanghai for Science and Technology, Shanghai 200093, China

²Key Laboratory of Medical Optical Instruments and Devices, Ministry of Education, University of Shanghai for Science and Technology, Shanghai 200093, China

³Department of Dermatology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

⁴Engineering Research Center of Optical Instruments and Systems, Ministry of Education, University of Shanghai for Science and Technology, Shanghai 200093, China

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Figures & Tables(10)

Fig. 1. System working schema

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Fig. 2. Schematic diagram of the software interaction interface

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Fig. 3. Tissue section stitching images. (a) Normal skin tissue section; (b) cSCC tissue section

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Fig. 4. Histological staining iamges. (a) Normal skin tissue; (b) cSCC tissue

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Fig. 5. Pseudocolor segmentation images. (a) Segmentation image of normal tissue at 600 nm; (b) segmentation image of normal tissue at 630 nm; (c) segmentation image of cSCC tissue at 600 nm; (d) segmentation image of cSCC tissue at 630 nm

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Fig. 6. Proportion of nuclear area within the window of normal tissue and cSCC tissue sections. (a) Normal tissue section; (b) cSCC tissue section

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Fig. 7. Distribution of nuclear-cytoplasmic ratio in skin tissue

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Fig. 8. Nuclear-cytoplasmic ratio statistical results

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Fig. 9. ROC curve

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Table 1. System performance parameters
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Table 1. System performance parameters
Parameter Value
Number of bands 13
Wavelength /nm 420‒680
Spectral resolution /nm 20
Spatial resolution /μm ≤0.4
Magnification 140
Translational table stroke /（mm×mm） 50×50

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Cheng Wang, Changxing Yang, Jiayi Yang, Qianqian Ge, Wenqiang Cai, Yuling Yan, Huazhong Xiang, Dawei Zhang, Xiaoqing Zhao. Pathological Analysis of Cutaneous Squamous Cell Carcinoma Based on Multispectral Microscopic Imaging[J]. Chinese Journal of Lasers, 2024, 51(15): 1507204

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