Acta Optica Sinica, Volume. 39, Issue 12, 1218001(2019)
Microscopy Autofocus Method Using Tilt Camera
Figures & Tables(14)
Fig. 2. Relationship between defocusing distance and focusing band horizontal shift of tilt plane image
Fig. 6. Schematic of calculation method for horizontal shift of focusing band of tilt plane image
Fig. 7. Tilt plane image and Gaussian fitting curve of sharpness-pixel scatter. (a) Tilt plane image; (b) ROI; (c) Gaussian fitting curve of sharpness-pixel scatter
Fig. 8. Calibration curve between defocusing distance and horizontal shift value of focusing band
Fig. 10. Testing results of operating range and defocusing distance error of the system. (a)(c)(e) Relationship between mean values of measured defocusing distance and test defocusing distance under different NA; (b)(d)(f) curves of defocusing distance error and repeatability error under different NA
Fig. 11. Average time-consumption of single-frame image processed with different sharpness evaluation functions
Table 1. Experimental data of calibration curve between defocusing distance and horizontal shift value of focusing band (parameters of objective lens: magnification is 10; NA is 0.3)
View tableTable 1. Experimental data of calibration curve between defocusing distance and horizontal shift value of focusing band (parameters of objective lens: magnification is 10; NA is 0.3)
Test number Defocusing distance interval / μm Grating value Defocusing distance ΔZ / μm Shift value ΔN 1 [-19,-17) -364 -18.20 1081.50 2 [-17,-15) -325 -16.25 969.89 3 [-15,-13) -280 -14.00 852.18 4 [-13,-11) -243 -12.15 745.47 5 [-11,-9) -200 -10.00 623.79 6 [-9,-7) -157 -7.85 501.64 7 [-7,-5) -117 -5.85 383.22 8 [-5,-3) -74 -3.70 265.63 9 [-3,-1) -33 -1.65 146.43 10 [-1,1) 0 0 -2.60 11 [1,3) 38 1.90 -87.02 12 [3,5) 78 3.90 -180.01 13 [5,7) 121 6.05 -277.05 14 [7,9) 160 8.00 -388.47 15 [9,11) 200 10.00 -505.82 16 [11,13) 235 11.75 -616.50 17 [13,15) 270 13.50 -737.47 18 [15,17) 305 15.25 -844.51 19 [17,19] 344 17.20 -961.49
Table 2. Experimental data sheet of system performance test (lens parameters of objective: magnification is 10; NA is 0.3)μm
View tableTable 2. Experimental data sheet of system performance test (lens parameters of objective: magnification is 10; NA is 0.3)μm
Zt Zm Ze σ Zt Zm Ze σ 28.08 29.73 1.65 0.40 -2.00 -1.94 0.06 0.12 26.03 25.77 -0.26 0.22 -3.98 -4.42 -0.44 0.16 24.00 22.98 -1.02 0.32 -6.08 -6.68 -0.60 0.20 21.95 21.25 -0.70 0.25 -7.95 -8.71 -0.76 0.32 19.97 19.45 -0.52 0.22 -10.03 -10.98 -0.95 0.30 18.03 17.71 -0.32 0.08 -12.02 -13.17 -1.15 0.17 16.00 15.76 -0.24 0.20 -14.04 -14.97 -0.93 0.29 13.98 13.57 -0.41 0.14 -15.97 -17.09 -1.12 0.11 12.05 11.56 -0.49 0.15 -17.99 -18.80 -0.81 0.30 10.00 9.98 -0.02 0.30 -20.00 -20.74 -0.74 0.10 8.07 8.13 0.06 0.18 -22.07 -22.67 -0.60 0.19 6.02 6.33 0.31 0.17 -24.06 -25.23 -1.17 0.38 3.99 4.30 0.31 0.13 -26.02 -29.11 -3.09 0.35 1.95 2.53 0.58 0.12 -27.93 -33.77 -5.84 0.66
Table 3. Comparison of system performance test data for 10× objective lens with different NAμm
View tableTable 3. Comparison of system performance test data for 10× objective lens with different NAμm
NA Operating range Zo Defocusing distance error Ze Maximum repeatability error σmax 0.25 44 [-1,1] 0.36 0.3 52 [-1.2, 0.6] 0.38 0.35 36 [-1,1.5] 0.33
Tools
Get Citation
Copy Citation Text
Yiqing Ye, Dingrong Yi, Yongzhen Zhang, Linghua Kong, Wei Jiang. Microscopy Autofocus Method Using Tilt Camera[J]. Acta Optica Sinica, 2019, 39(12): 1218001
Paper Information
Category: Microscopy
Received: May. 31, 2019
Accepted: Sep. 2, 2019
Published Online: Dec. 6, 2019
The Author Email: Yi Dingrong (yidr@hqu.edu.cn)
© Copyright 2018-2021 | Chinese Laser Press.
All Rights Reserved 沪ICP备15018463号-20