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Laser & Optoelectronics Progress, Volume. 61, Issue 20, 2011003(2024)

Research on Differential Compressed Imaging-Based Structured Illumination Super-Resolution Microscopy Technique (Invited)

Bozhang Cheng1, Yunhua Yao1、**, Yilin He1, Zhengqi Huang1, Yu He1, Mengdi Guo1, Dalong Qi1, Yuecheng Shen1, Zhenrong Sun1, and Shian Zhang1,2,3、*
Author Affiliations
  • 1State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China
  • 2Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, Shanxi , China
  • 3Joint Research Center of Light Manipulation Science and Photonic Integrated Chip of East China Normal University and Shandong Normal University, East China Normal University, Shanghai 200241, China
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    AbstractGet PDF(in Chinese)
    Figures&Tables (7)
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    References (34)
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    Figures & Tables(7)
    Image acquisition models of CISIM and DCISIM. (a) CISIM; (b) DCISIM

    Fig. 1. Image acquisition models of CISIM and DCISIM. (a) CISIM; (b) DCISIM

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    The comparison of spatial spectra of the images. (a) Origin image; (b) wide field image; (c) compressed image by CISIM; (d) differential image by DCISIM; (e)‒(h) corresponding spatial frequency spectra of (a)‒(d)

    Fig. 2. The comparison of spatial spectra of the images. (a) Origin image; (b) wide field image; (c) compressed image by CISIM; (d) differential image by DCISIM; (e)‒(h) corresponding spatial frequency spectra of (a)‒(d)

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    Framework of the image reconstruction algorithm for DCISIM

    Fig. 3. Framework of the image reconstruction algorithm for DCISIM

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    Comparison of CISIM and DCISIM reconstruction results for several biological structures. (a) Comparison of reconstruction images; (b) intensity distribution of different images

    Fig. 4. Comparison of CISIM and DCISIM reconstruction results for several biological structures. (a) Comparison of reconstruction images; (b) intensity distribution of different images

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    Comparison of CISIM and DCISIM reconstruction results based on SIM experimental data. (a) Comparison of reconstruction images; (b) intensity distribution of different images

    Fig. 5. Comparison of CISIM and DCISIM reconstruction results based on SIM experimental data. (a) Comparison of reconstruction images; (b) intensity distribution of different images

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    Comparison of image reconstruction quality for DCISIM under different noise intensities. (a) Comparison of reconstruction images; (b) intensity distribution of different images; (c) variation in PSNR and SSIM with noise intensity of reconstruction images

    Fig. 6. Comparison of image reconstruction quality for DCISIM under different noise intensities. (a) Comparison of reconstruction images; (b) intensity distribution of different images; (c) variation in PSNR and SSIM with noise intensity of reconstruction images

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    • Table 1. Image quality evaluation indicators of three set reconstruction results

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      Table 1. Image quality evaluation indicators of three set reconstruction results

      IndicatorStructureWide fieldCISIMDCISIM
      PSNR /dBMicrotubule associated protein in neuron27.7337.6341.39
      Microtubule in COS-7 cell24.4339.7843.33
      Trio protein in Swiss 3T3 cell15.5117.9720.56
      SSIMMicrotubule associated protein in neuron0.92270.98630.9939
      Microtubule in COS-7 cell0.88030.99260.9935
      Trio protein in Swiss 3T3 cell0.47780.71680.8466
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    Bozhang Cheng, Yunhua Yao, Yilin He, Zhengqi Huang, Yu He, Mengdi Guo, Dalong Qi, Yuecheng Shen, Zhenrong Sun, Shian Zhang. Research on Differential Compressed Imaging-Based Structured Illumination Super-Resolution Microscopy Technique (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(20): 2011003

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    Paper Information

    Category: Imaging Systems

    Received: Jun. 28, 2024

    Accepted: Aug. 1, 2024

    Published Online: Nov. 4, 2024

    The Author Email: Yao Yunhua (yhyao@lps.ecnu.edu.cn), Zhang Shian (sazhang@phy.ecnu.edu.cn)

    DOI:10.3788/LOP241570

    CSTR:32186.14.LOP241570

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology