Advanced Photonics Nexus, Volume. 2, Issue 2, 026011(2023)

Noncontact photoacoustic lipid imaging by remote sensing on first overtone of the C-H bond

Guyue Hu1, Qiao Ran2, Beth Wing Lam So3, Mingsheng Li1, Jiawei Shi1, Xin Dong1, Jiqiang Kang1,4、*, and Kenneth K. Y. Wong1,4、*
Author Affiliations
  • 1The University of Hong Kong, Faculty of Engineering, Department of Electrical and Electronic Engineering, Hong Kong, China
  • 2The University of Hong Kong, School of Biological Sciences, Hong Kong, China
  • 3The University of Hong Kong, School of Biomedical Sciences, LKS Faculty of Medicine, Pokfulam, Hong Kong, China
  • 4Hong Kong Science Park, Advanced Biomedical Instrumentation Centre, Hong Kong, China
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    Figures & Tables(4)
    Scheme of the PARS setup. CIR, circulator; COL, collimator; DM, dichroic mirror; BS, beam splitter; OL, objective lens; CCD, charge-coupled device; C/L, C/L band filter; PD, photodiode; DC, DC block; AMP, amplifier.
    Imaging results of carbon fibers. (a) PARS image of carbon fiber networks. (b) Typical PARS signal of a single carbon fiber in the time domain. (c) Lateral resolution of the PARS system by scanning a carbon fiber, resulting in an FWHM of 1.62 μm. (d) Axial resolution of the system by an A-scan of a carbon fiber, with an FWHM of 30.25 μm.
    PARS images of phantom samples. (a) PARS image of a droplet of olive oil. (b) PARS image of solid butter with a specific branch-like pattern. (c) PARS signals of oil and butter in the time domain. (d) frequency responses of the PARS signals.
    PARS images of biological samples. (a) PARS image of the unlabeled C. Elegans. The right upper corner figure is the sample under bright-field microscopy. The yellow window shows the enlarged image with a smaller step size, which is displayed as (b). (c) PARS image of the mouse brain slice, of which the white window shows high contrasts of the tissue.
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    Guyue Hu, Qiao Ran, Beth Wing Lam So, Mingsheng Li, Jiawei Shi, Xin Dong, Jiqiang Kang, Kenneth K. Y. Wong, "Noncontact photoacoustic lipid imaging by remote sensing on first overtone of the C-H bond," Adv. Photon. Nexus 2, 026011 (2023)

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    Paper Information

    Category: Research Articles

    Received: Dec. 22, 2022

    Accepted: Mar. 3, 2023

    Published Online: Mar. 29, 2023

    The Author Email: Jiqiang Kang (jiqiangkang@gmail.com), Kenneth K. Y. Wong (kywong@eee.hku.hk)

    DOI:10.1117/1.APN.2.2.026011

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