Spectral domain polarization-sensitive optical coherence tomography (SDPS-OCT) is a depth-resolved polarization-sensitive interferometry which integrates polarization optics into spectral domain optical coherence tomography (SD-OCT). This configuration can obtain birefringence information of samples and improve the imaging speed. In this paper, horizontally polarized light is used to replace natural light of the source. Then, right-rotated circularly polarized light is the incident sample light. To obtain two orthogonal components of the polarized interferogram, the reflected light of the reference arm is set to be 45O linearly polarized light. These two components are acquired by two spectrometers synchronously. The system was employed to achieve 12.8-\mum axial resolution and 4.36-\mum transverse resolution. We have imaged in vitro chicken tendon and muscle tissues with these system.