Laser & Optoelectronics Progress, Volume. 62, Issue 13, 1300004(2025)

Current Research on Bloodstain Detection Using Spectroscopic Techniques

Huihui Ren1, Xinyu Zhang1, Fanfan Cheng3, Yuran Qiu3, Junda Ma3, Jianqiang Zhang3, Jiaquan Wu1, Hang Chen1, Xiaoquan Lu1, and Qifu Yang2、*
Author Affiliations
  • 1College of Science, Kunming University of Science and Technology, Kunming 650500, Yunnan , China
  • 2Nanophysics and Devices Laboratory, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
  • 3College of Criminal Investigation, Yunnan Police College, Kunming 650223, Yunnan , China
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    References(70)

    [1] Zhao M, Yu J, Liu L et al. Thoughts on improving the quality of experimental teaching of forensic evidence[J]. Journal of Zunyi Medical University, 34, 441-443(2011).

    [2] Feng X P. Study on the morphology and characteristics detection of bloodstains based on hyperspectral imaging technology[D], 8-9(2013).

    [3] Hu N N. Research on blood stain detection based on hyper-spectral imaging technology[D], 2(2019).

    [4] Cen Y. Research on safety testing technology and safety control measures of food additives[J]. China Food Safety Magazine, 163-165(2024).

    [5] Xie Y, Shi Z Y, Pang H D et al. Research on microplastics pollution and development of its detection methods[J]. Environmental Science and Management, 49, 105-110(2024).

    [6] Chu X L[M]. Chemometric methods in modern spectral analysis, 3-14(2022).

    [7] Zhao Y. Exploration on the analysis method of bloodstain age based on clustering algorithm[D], 1-64(2022).

    [8] Li M, Yuan Z J. Analytical chemistry in bloodstain examination[J]. Chinese Journal of Chemical Education, 42, 11-16(2021).

    [9] Yan X Y, Zhang J Z, Jin J et al. Application progress of bloodstain presumptive test reagents to detect bloodstains at fire scenes[J]. Chinese Journal of Chemical Education, 44, 7-14(2023).

    [10] Chen W B, Huo S H, Zhu K W. Experimental study on potential blood stains and influencing factors of luminol and tetramethylbenzidine[J]. Guangdong Gongan Keji, 22, 23-27(2014).

    [11] Gao W Y, Pan J C. Study on the sensitivity and false positive reaction of blue star reagent[J]. Journal of Railway Police College, 28, 105-109(2018).

    [12] Rong Z W, Du X J, Chang B N. Methods for fixing and developing blood handprints[J]. Forensic Science and Technology, 22-23(1980).

    [13] She X, Huang Y P, Chen W J et al. Research on the method of latent blood stains in crime scene[J]. Journal of Jiangsu Police Institute, 29, 39-44(2014).

    [14] Zhu C H, Zheng D L, Ni Y Z et al. DNA quantification of blood samples pre-treated with pyramidon[J]. Journal of Forensic Medicine, 30, 188-190(2014).

    [15] Kan C, Zhu X J, Cheng L et al. Obsolescence degree of bloodstain in non-porous object detected by micro spectrophotometer method[J]. Journal of Nature Science, 4, 145-155(2016).

    [16] Li Y Q, Zhao G S, Chen J et al. The conclusive test of hemochromogen by micro spectroscopic method[J]. Chinese Journal of Forensic Medicine, 31, 601-604(2016).

    [17] Guo X D. Extraction and purification process optimization and industrialization of yak blood hemin[D], 1-2(2022).

    [18] Dong Y, Yu X H. Analysis and discussion of methods for inferring time of death[J]. Guangdong Gongan Keji, 26, 72-73(2018).

    [19] Zhou T, Fu L P. The application of digital signal processing technology in chemical analysis[J]. Shandong Chemical Industry, 53, 125-127(2024).

    [20] Yang Z C. Study on detection method of hazardous substances in milk based on surface-enhanced Raman spectroscopy[D], 8(2023).

    [22] Shaine M L. Optimization of the forensic identification of blood using surface-enhanced Raman spectroscopy[D], 1-77(2020).

    [23] Thomas J M. The detection and identification of blood on interfering substrates using Raman spectroscopy and Hamand for forensic purposes[D], 1-22(2021).

    [26] Shi Q H, Ren X L. Research advance on detection methods of salmonella[J]. Meat Research, 23, 75-79(2009).

    [31] Huang W, Gao L M, Guo W W et al. Analysis into functional data of spectral images from bloodstains of human and two species of animal[J]. Forensic Science and Technology, 46, 551-558(2021).

    [32] Zhuang Y, Gao S H, Xie F et al. Identifying bloodstain species using hyperspectral imaging[J]. Laser & Optoelectronics Progress, 59, 1630001(2022).

    [35] Dickler H. Discrimination between human and non-human blood using Raman spectroscopy and a self-reference algorithm for forensic purposes: method expansion and validation[D], 1-14(2022).

    [36] Bi F L, Wang W, Qi Y Y et al. Rapid identification of bloodstain based on near infrared spectroscopy and extreme learning machine algorithm[J]. Forensic Science and Technology, 49, 507-513(2024).

    [38] Liang Y, Wu J Q, Zeng Q et al. Rapid identification of the species of bloodstain based on near infrared spectroscopy and convolutional neural network-support vector machine algorithm[J]. Journal of the Brazilian Chemical Society, 35, 1-6(2024).

    [40] Zhang L J. The body’s circadian rhythms has an influence on blood indicators in the exercise[D], 6-7(2014).

    [43] Gao Q Y, Gao S M. Authentication of age of bloodstains using UV visible reflection spectrum[J]. Spectroscopy and Spectral Analysis, 35, 2221-2224(2015).

    [44] Yan L Q, Gao Y. Determination of bloodstain age by UV visible integrating sphere reflection spectrum[J]. Journal of Forensic Medicine, 32, 326-328(2016).

    [45] Hu Q L, Liu J W, Feng A Q et al. Application of photometric analysis of blood stain to evidence analysis[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 45, 70-72(2009).

    [47] Li H K, Cao Q F, Shi H et al. Comparative analysis of main properties of thermal sublimation transfer paper at home and abroad[C], 94-101(2024).

    [48] Duan Y Q, Liao H H, Zheng C et al. Identification of selenium-enriched green tea based on improved kmeans algorithm[J]. Journal of Hubei Minzu University (Natural Science Edition), 37, 445-448, 462(2019).

    [49] Cao H, Zhang J D. Analysis of digital color space model in ship video surveillance[J]. Journal of Dalian Maritime University, 38, 108-110, 116(2012).

    [50] Xu Y W, Sun S R, Zhang J C et al. Research on the Marangoni effect in droplet explosions[J]. College Physics, 43, 44-50(2024).

    [52] Feng S S. Research on automatic blood stain classification based on drip spatter and spatter patterns[D], 10-11(2019).

    [55] Zheng J L, Wen H Y, Zhang B et al. Development of integrated device of trace bloodstains imaging and age analysis[J]. Journal of Forensic Medicine, 35, 230-233, 239(2019).

    [57] Qian Z L, Li X, Jia Z J et al. Research on bloodstain age inference based on Image J software[J]. Chinese Journal of Forensic Medicine, 38, 43-47(2023).

    [59] Li B, Beveridge P, O’Hare W T et al. The estimation of the age of a blood stain using reflectance spectroscopy with a microspectrophotometer, spectral pre-processing and linear discriminant analysis[J]. Forensic Science International, 212, 198-204(2011).

    [62] Sun W, Liu H C, Liu J K et al. Age imaging by hyperspectral estimation of bloodstains technology based on partial least squares regression[J]. Applied Chemical Industry, 51, 273-276, 285(2022).

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    Huihui Ren, Xinyu Zhang, Fanfan Cheng, Yuran Qiu, Junda Ma, Jianqiang Zhang, Jiaquan Wu, Hang Chen, Xiaoquan Lu, Qifu Yang. Current Research on Bloodstain Detection Using Spectroscopic Techniques[J]. Laser & Optoelectronics Progress, 2025, 62(13): 1300004

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    Paper Information

    Category: Reviews

    Received: Nov. 25, 2024

    Accepted: Dec. 25, 2024

    Published Online: Jul. 16, 2025

    The Author Email: Qifu Yang (2633151251@qq.com)

    DOI:10.3788/LOP242314

    CSTR:32186.14.LOP242314

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