Laboratory Animal and Comparative Medicine, Volume. 45, Issue 4, 432(2025)

Exploration of Rat Fetal Lung Tissue Fixation Methods

LIU Liyu, JI Bo, LIU Xiaoxuan, FANG Yang, ZHANG Ling, GUO Tingting, QUAN Ye, LI Hewen, and LIU Yitian*
Author Affiliations
  • School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
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    References(12)

    [1] [1] WARBURTON D, EL-HASHASH A, CARRARO G, et al. Lung organogenesis[J]. Curr Top Dev Biol, 2010, 90: 73-158. DOI: 10.1016/s0070-2153(10)90003-3.

    [2] [2] SHI H J, ZHANG S J, LAI F P, et al. The establishment and severity assessment of ultrasound-guided prenatal broncho-pulmonary dysplasia model in rat[J]. Sci Rep, 2024, 14(1): 18133. DOI: 10.1038/s41598-024-69051-w.

    [3] [3] PEERS DE NIEUWBURGH M, HUNT M, CHANDRASEKARAN P, et al. Chronic hypoxia in an Extrauterine Environment for Neonatal Development impairs lung development[J]. Am J Respir Cell Mol Biol, 2025, 72(4): 441-452. DOI: 10.1165/rcmb.2024-0012OC.

    [4] [4] QU W S, YIN J Y, WANG H M, et al. A simple method for the formalin fixation of lungs in toxicological pathology studies[J]. Exp Toxicol Pathol, 2015, 67(10): 533-538. DOI: 10.1016/j.etp.2015.08.002.

    [10] [10] STACEY M T, JOSEPH B, WILLIAM J J, et al. Air-inflation of murine lungs with vascular perfusion-fixation[J]. J Vis Exp, 2021 (168): 10.3791/62215. DOI: 10.3791/62215.

    [11] [11] MARK R W. The hematoxylin and eosin stain in anatomic pathology-An often-neglected focus of quality assurance in the laboratory[J]. Semin Diagn Pathol, 2019, 36(5): 303-311. DOI: 10.1053/j.semdp.2019.06.003.

    [13] [13] LEARY S, UNDERWOOD W, ANTHONY R, et al. AVMA Guidelines for the euthanasia of animals: 2020 Edition[M]. Schaumburg: American Veterinary Medical Association, 2020.

    [15] [15] MATT U, HEROLD S. Traffic jam in lung capillaries: inter-organ communication impedes gas exchange after acute kidney injury[J]. J Clin Invest, 2025, 135(10): e192917. DOI: 10.1172/JCI192917.

    [16] [16] DHANYA M, NICOLA P S. Lung development[J]. Semin Pediatr Surg, 2015, 24(4): 152-155. DOI: 10.1053/j.sempedsurg.2015.01.011.

    [17] [17] MICHELE G, CHIARA G, WILLIAM W S, et al. Current definitions of the breathing cycle in alveolar breath-by-breath gas exchange analysis[J]. Am J Physiol Regul Integr Comp Physiol, 2023, 325(5): R433-R445. DOI: 10.1152/ajpregu.00065.2023.

    [18] [18] KUPPEVELT T H, ROBBSESOM A A, VERSTEEG E M, et al. Restoration by vacuum inflation of original alveolar dimensions in small human lung specimens[J]. Eur Respir J, 2000, 15(4): 771-777. DOI: 10.1034/j.1399-3003.2000.15d23.x.

    [21] [21] BRABER S, VERHEIJDEN K T, HENRICKS P J, et al. A comparison of fixation methods on lung morphology in a murine model of emphysema[J]. Am J Physiol Lung Cell Mol Physiol, 2010, 299(6): L843-L851. DOI: 10.1152/ajplung.00192.2010.

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    LIU Liyu, JI Bo, LIU Xiaoxuan, FANG Yang, ZHANG Ling, GUO Tingting, QUAN Ye, LI Hewen, LIU Yitian. Exploration of Rat Fetal Lung Tissue Fixation Methods[J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 432

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    Paper Information

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    Received: Apr. 3, 2025

    Accepted: Sep. 10, 2025

    Published Online: Sep. 10, 2025

    The Author Email: LIU Yitian (lytbucm@163.com)

    DOI:10.12300/j.issn.1674-5817.2025.053

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