Study on Selecting Characterization Parameters of Viable Algae Cells Number in Ballast Water Based on Chlorophyll Fluorescence Kinetics

Hui Hua; Gaofang Yin; Nanjing Zhao; Tingting Gan; Min Chen; Lu Wang; Peilong Qi; Xiang Wang; Xiaoling Zhang; Li Fang; Ruifang Yang; Ming Dong; Zhihao Chen; Renqing Jia; Zhichao Ding; Jianguo Liu

doi:10.3788/AOS202141.0617001

Acta Optica Sinica, Volume. 41, Issue 6, 0617001(2021)

Study on Selecting Characterization Parameters of Viable Algae Cells Number in Ballast Water Based on Chlorophyll Fluorescence Kinetics

Hui Hua^1,2,3, Gaofang Yin^1,3、*, Nanjing Zhao^1,3、**, Tingting Gan^1,3, Min Chen^1,2,3, Lu Wang^1,2,3, Peilong Qi^1,2,3, Xiang Wang^1,2,3, Xiaoling Zhang⁴, Li Fang^1,3, Ruifang Yang^1,3, Ming Dong^1,2,3, Zhihao Chen^1,2,3, Renqing Jia^1,2,3, Zhichao Ding^1,2,3, and Jianguo Liu^1,3

Author Affiliations

¹Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China

²University of Science and Technology of China, Hefei, Anhui 230026, China

³Anhui Provincial Key Laboratory of Optical Monitoring Technology for Environment, Hefei, Anhui 230031, China

⁴Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China

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Figures & Tables(9)

Fig. 1. Change trend of photosynthetic fluorescence parameters with the increase of dead cells number

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Fig. 2. Change trend of photosynthetic fluorescence parameters with the increase of CDOM concentration

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Fig. 3. Changes of photosynthetic fluorescence parameters with viable algae cells number in different diluents. (a) F_m; (b) F₀; (c) F_v; (d) [RCII]

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Fig. 4. T-test results between the same type photosynthetic fluorescence parameters with seawater as a diluent and those with other fluorescence interference solution as a diluent

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Fig. 5. Quantitative relationship of photosynthetic fluorescence parameters to viable algae cells number. (a) F_v; (b) F₀; (c) F_m; (d) [RCII]

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Fig. 6. Trend diagram of photosynthetic fluorescence parameters and viable algae cells number under DCMU stress

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Table 1. Photosynthetic fluorescence parameters acquired by FRRf

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Table 1. Photosynthetic fluorescence parameters acquired by FRRf

Parameter	Measurement or derivation	Physiological significance
F_m	At the asymptote of a single turnover (ST) pulse	Maximum yields of chlorophyll a fluorescencemeasured after dark adaptation
F₀	Extrapolation to t=0 from a single turnover (ST) pulse	Minimum yields of chlorophyll a fluorescencemeasured after dark adaptation
F_v	F_m-F₀	Variable fluorescence
σ_PSII	Fluorescence kinetic curve (the rate of the fluorescencerise is proportional to the functional absorption crosssection of PSII (σ_PSII))	Functional absorption cross section for PSII
[RCII]	F₀/σ_PSII	Effective photosynthetic reaction center concentration

Table 2. Correlation analysis between the photosynthetic fluorescence parameters and viable algae cells number in diluted seawater and mixture

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Table 2. Correlation analysis between the photosynthetic fluorescence parameters and viable algae cells number in diluted seawater and mixture

Fluorescenceparameter	Correlationcoefficient(diluted seawater)	Correlationcoefficient(mixture)
F_m	0.989^**	-0.692
F₀	0.995^**	-0.914^**
F_v	0.955^*	0.955^**
[RCII]	0.997^*	-0.845
Note:^** represents p<0.01,^* represents p<0.05.

Table 3. Correlation analysis between photosynthetic fluorescence parameters and viable algae cells number under the stress of DCMU
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Table 3. Correlation analysis between photosynthetic fluorescence parameters and viable algae cells number under the stress of DCMU
Parameter Correlation coefficient
F_m -0.997^**
F₀ -0.999^**
F_v 0.986^**
[RCII] -0.888^*
Note:^** represents p<0.01,^* represents p<0.05.

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Hui Hua, Gaofang Yin, Nanjing Zhao, Tingting Gan, Min Chen, Lu Wang, Peilong Qi, Xiang Wang, Xiaoling Zhang, Li Fang, Ruifang Yang, Ming Dong, Zhihao Chen, Renqing Jia, Zhichao Ding, Jianguo Liu. Study on Selecting Characterization Parameters of Viable Algae Cells Number in Ballast Water Based on Chlorophyll Fluorescence Kinetics[J]. Acta Optica Sinica, 2021, 41(6): 0617001

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Paper Information

Category: Medical Optics and Biotechnology

Received: Sep. 2, 2020

Accepted: Nov. 11, 2020

Published Online: Apr. 7, 2021

The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn), Zhao Nanjing (njzhao@aiofm.ac.cn)

DOI:10.3788/AOS202141.0617001

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Photosynthetic fluorescence parameters acquired by FRRf

Table 1. Photosynthetic fluorescence parameters acquired by FRRf

Table 2. Correlation analysis between the photosynthetic fluorescence parameters and viable algae cells number in diluted seawater and mixture

Table 2. Correlation analysis between the photosynthetic fluorescence parameters and viable algae cells number in diluted seawater and mixture

Table 3. Correlation analysis between photosynthetic fluorescence parameters and viable algae cells number under the stress of DCMU

Table 3. Correlation analysis between photosynthetic fluorescence parameters and viable algae cells number under the stress of DCMU