Multispectral Analysis of Interaction Between Catechins and Egg Yolk Immunoglobulin and the Change of Bacteriostasis

Meng-jun ZHANG; Li-li LIU; Xie-li YANG; Jing-fang GUO; Hao-yang WANG

doi:10.3964/j.issn.1000-0593(2022)07-2297-07

Spectroscopy and Spectral Analysis, Volume. 42, Issue 7, 2297(2022)

Multispectral Analysis of Interaction Between Catechins and Egg Yolk Immunoglobulin and the Change of Bacteriostasis

Meng-jun ZHANG1,*... Li-li LIU1,1; *;, Xie-li YANG2,2;, Jing-fang GUO1,1; and Hao-yang WANG1,1; |Show fewer author(s)

¹1. College of Food and Bioengineering, National Experimental Teaching Demonstration Center for Food Processing and Security, Food Microbiology Engineering Technology Research Center of Henan Province, Research and Utilization of Functional Food Resources Science and Technology Innovation Team of Henan Provincial Department of Education, Luoyang 471023, China

²2. LIZHENG College, Henan University of Science and Technology, Luoyang 471023, China

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Figures & Tables(11)

Fig. 1. Ultraviolet-visible spectrum of different concentrations of catechins binding to IgY
The mass concentration of IgY was 0.5 mg·mL^-1; The mass concentrations of 1 to 5 catechins were 0, 6.25, 12.5, 18.75, 25 μg·mL^-1, respectively, as shown in Figure 2

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Fig. 2. Fluorescence spectra of different concentrations of catechins and different temperature binding to IgY
(a): 18 ℃; (b): 25 ℃; (c): 37 ℃

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Fig. 3. Stern-Volmer plots of IgY quenched by catechinat different temperatures

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Fig. 4. Logarithmic diagram of IgY-catechin complexes at different temperatures

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Fig. 5. Fourier transform infrared spectra of IgY and IgY-catechins complex

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Fig. 6. Antibacterial effect of IgY, mixture and IgY-catechin complex

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Table 1. Quenching rate constants and correlation coefficients of catechin and IgY
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Table 1. Quenching rate constants and correlation coefficients of catechin and IgY
温度/
K K_sv/
(L·mol^-1) K_q/
[L·(mol·s)^-1] R²
291 1.053×10⁴ 1.053×10¹² 0.989 9
298 0.934×10⁴ 0.934×10¹² 0.982 0
310 0.818×10⁴ 0.818×10¹² 0.992 6

Table 2. Apparent binding constant, number of binding sites and linear correlation coefficient of catechin-IgY complex
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Table 2. Apparent binding constant, number of binding sites and linear correlation coefficient of catechin-IgY complex
温度/K K_A/(L·mol^-1) n R²
291 2.455×10⁴ 1.089 0.979
298 1.694×10⁴ 1.067 0.961
310 1.183×10⁴ 1.042 0.984

Table 3. Thermodynamic parameters of catechin-IgY complex
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Table 3. Thermodynamic parameters of catechin-IgY complex
温度/
K ΔH/
(kJ·mol^-1) ΔG/
(kJ·mol^-1) ΔS/
(J·K^-1)
291 -24.456 -47.282
298 -38.215 -24.125 -47.282
310 -24.171 -45.303

Table 4. The relative content of secondary structure of protein in amide Ⅰ band of IgY and IgY-catechin complex
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Table 4. The relative content of secondary structure of protein in amide Ⅰ band of IgY and IgY-catechin complex
样品 β-折叠
/% 无规卷曲
/% α-螺旋
/% β-转角
/%
IgY 42.81 23.12 19.68 14.39
复合物 42.53 17.84 25.16 14.47

Table 5. Bacteriostatic rate of IgY, mixture and IgY-catechin complex

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Table 5. Bacteriostatic rate of IgY, mixture and IgY-catechin complex

菌种	样品	抑菌率/%
菌种	样品	0.025 mg·mL^-1	0.05 mg·mL^-1	0.1 mg·mL^-1	0.15 mg·mL^-1	0.2 mg·mL^-1
	IgY	22.08±0.86 b	23.98±0.44 b	29.88±0.21 b	30.75±0.11 b	32.11±1.15 b
大肠杆菌	混合物	27.60±0.68 a	29.07±1.03 a	29.48±0.73 b	31.17±0.17 b	34.26±0.40 b
	复合物	22.33±0.86 b	24.38±0.82 b	34.14±0.21 a	35.41±0.62 a	37.83±0.76 a
	IgY	24.97±1.27 c	24.85±0.91 c	26.99±1.13 c	41.85±1.86 c	54.49±1.53 c
金黄色葡萄球菌	混合物	61.58±1.20 b	67.19±0.29 b	68.01±0.13 b	70.46±1.16 b	74.12±0.25 b
	复合物	68.29±0.30 a	72.16±0.83 a	75.06±0.49 a	79.15±0.29 a	80.79±1.12 a

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Meng-jun ZHANG, Li-li LIU, Xie-li YANG, Jing-fang GUO, Hao-yang WANG. Multispectral Analysis of Interaction Between Catechins and Egg Yolk Immunoglobulin and the Change of Bacteriostasis[J]. Spectroscopy and Spectral Analysis, 2022, 42(7): 2297

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Paper Information

Category: Orginal Article

Received: Jun. 13, 2021

Accepted: --

Published Online: Nov. 16, 2022

The Author Email: ZHANG Meng-jun (18438612672@163.com)

DOI:10.3964/j.issn.1000-0593(2022)07-2297-07

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Quenching rate constants and correlation coefficients of catechin and IgY

Table 1. Quenching rate constants and correlation coefficients of catechin and IgY

Table 2. Apparent binding constant, number of binding sites and linear correlation coefficient of catechin-IgY complex

Table 2. Apparent binding constant, number of binding sites and linear correlation coefficient of catechin-IgY complex

Table 3. Thermodynamic parameters of catechin-IgY complex

Table 3. Thermodynamic parameters of catechin-IgY complex

Table 4. The relative content of secondary structure of protein in amide Ⅰ band of IgY and IgY-catechin complex

Table 4. The relative content of secondary structure of protein in amide Ⅰ band of IgY and IgY-catechin complex

Table 5. Bacteriostatic rate of IgY, mixture and IgY-catechin complex

Table 5. Bacteriostatic rate of IgY, mixture and IgY-catechin complex

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