Spectroscopy and Spectral Analysis, Volume. 42, Issue 8, 2423(2022)

Detection of Melamine and Cyromazine in Raw Milk by Aptamer-Facilitated Gold Nanoparticle-Based Logic Gates

Cheng QIAN1,*... Bo WANG1,1;, Xue-lian FEI1,1;, Pan-cheng YIN1,1;, Bo-tao HUANG2,2;, Hai-bo XING1,1; *; and Xiao-jun HU1,1; *; |Show fewer author(s)
Author Affiliations
  • 11. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China
  • 22. Shanghai Academy of Environmental Sciences, Shanghai 200233, China
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    Figures & Tables(13)
    AND logic gate 1 design and fabrication(a) Scheme of AND logic gate 1 in the presence of no input (0, 0), Mel alone (1, 0), CTAB alone (0, 1) and both Mel and CTAB (1, 1);(b) Truth table; (c) Optical photographs; (d) Values of absorption ration (A650/A526) for the AND logic gate 1
    Transmission electron microscopy (TEM) images of AND logic gate 1(a): (0, 0); (b): (1, 0); (c): (0, 1); (d): (1, 1)
    (a) The effect of the pH on melamine detection; (b) The effect of aptamer concentration on melamine detection, the concentrations of aptamer were from 0 to 36 nmol·L-1; (c) The response time; (d) The optimization of temperature
    Sensitivity of the biosensor for melamine detectionThe concentrations of melamine are 0.00, 0.05, 0.10, 0.15, 0.20, 0.30, 0.40, 0.50, 0.60, 0.70, 0.80, 0.90, 1.00, 1.10, 1.20, 1.30, 1.40, 1.50, 1.60, 1.70, 1.80, 1.90, 2.00 mg·L-1. Inset: At low concentrations of melamine the data were fitted to a linear plot
    AND logic gate 2 design and fabrication(a) Scheme of AND logic gate 2 in the presence of no input (0, 0), Cyr alone (1, 0), CTAB alone (0, 1) and both Cyr and CTAB (1, 1); (b) Truth table; (c) Optical photographs; (d) Values of absorption ration (A650/A526) for the AND logic gate 2
    Transmission electron microscopy (TEM) images of AND logic gate 2(a): (0, 0); (b): (1, 0); (c): (0, 1); (d): (1, 1)
    (a) The effect of aptamer concentration on cyromazine detection, the concentrations of aptamer were from 0 to 36 nmol·L-1; (b) The effect of the CTAB concentration on cyromazine detection, the concentrations of CTAB were from 0.6 to 3.0 μmol·L-1; (c) The effect of the time on cyromazine detection, the time were from 0 to 16 min
    Sensitivity of the biosensor for cyromazine detectionThe concentrations of cyromazine are 0.0, 0.05, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, 0.40, 0.45, 0.50, 0.60, 0.70, 0.80, 0.90, 1.0, 1.5, 2.0 mg·L-1. Inset: At low concentrations of cyromaizne the data were fitted to a linear plot
    Selectivity of the assay for cyromazine detection over other veterinary drugs concentrations: cyromazine, ammelide, ammeline, aniline, L-tyrosine, cyanuric acid, L-lysine monoacetate, L-lysine monohydrochloride (500 μg·L-1). The concentration of Tcy1 is 15 nmol·L-1
    • Table 1. Experimental instruments

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      Table 1. Experimental instruments

      仪器名称型号/规格生产厂家/供货商
      离心机TD5M-WS上海卢湘仪离心机仪器有限公司
      恒温振荡器THZ-98C上海一恒科学仪器有限公司
      集热式恒温加热磁力搅拌器DF-101S巩义市予华仪器有限责任公司
      磁力搅拌器CJ-78-1上海跃进医疗器械有限公司
      pH计PB-10赛多利斯(德国)
      移液器1 mL/5 mL/10 mL赛多利斯(德国)
      电子天平千分之一赛多利斯(德国)
      高速混匀器XK80-A江苏新康医疗器械有限公司
      超声波清洗器KS-7200E昆山洁力美超声仪器有限公司
      电热恒温鼓风干燥箱DHG101-2上海赫田科学仪器有限公司
      冰箱BCD-321W博西华家用电器有限公司
      紫外可见光分光光度计UV-3500上海美谱达仪器有限公司
    • Table 2. Experimental reagents

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      Table 2. Experimental reagents

      试剂名称纯度生产厂家/供货商
      氯金酸、柠檬酸三钠分析纯上海化学试剂有限公司(中国上海)
      十六烷基三甲基溴化铵(CTAB)、氨苯胺、氨苯胺、苯胺、L-酪氨酸、三聚氰酸、L-赖氨酸、L-盐酸赖氨酸、环丙氨嗪、三聚氰胺分析纯西格玛奥德里奇(上海)贸易有限公司(Sigma-Aldrich)
      原料奶附近的一个养牛场
      去离子水泰坦科技
    • Table 3. Application of AND gate 1 and HPLC-MS to detect the melamine in raw milk samples spiked with different amounts of melamine

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      Table 3. Application of AND gate 1 and HPLC-MS to detect the melamine in raw milk samples spiked with different amounts of melamine

      样品HPLC-MSAND逻辑开关1
      添加值/
      (mg·L-1)
      检测结果/
      (mg·L-1)
      检测结果/
      (mg·L-1)
      回收率/
      %
      牛奶10.200 00.201 1±0.0010.196 7±0.00298.35
      牛奶20.500 00.502 1±0.0020.514 5±0.002102.9
      牛奶31.000 01.028 0±0.0020.961 6±0.00296.16
    • Table 4. Application of AND gate 2 and HPLC-MS to detect the cyromazine in raw milk samples spiked with different amounts of cyromaizne

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      Table 4. Application of AND gate 2 and HPLC-MS to detect the cyromazine in raw milk samples spiked with different amounts of cyromaizne

      样品HPLC-MSAND逻辑开关1
      添加值/
      (mg·L-1)
      检测量/
      (mg·L-1)
      添加值/
      (mg·L-1)
      回收率/
      %
      牛奶10.200 00.201 2±0.0020.197 898.90
      牛奶20.300 00.303 1±0.0040.311 4103.8
      牛奶30.500 00.501 7±0.010.510 3102.1
      牛奶41.000 00.998 7±0.011.116 0111.6
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    Cheng QIAN, Bo WANG, Xue-lian FEI, Pan-cheng YIN, Bo-tao HUANG, Hai-bo XING, Xiao-jun HU. Detection of Melamine and Cyromazine in Raw Milk by Aptamer-Facilitated Gold Nanoparticle-Based Logic Gates[J]. Spectroscopy and Spectral Analysis, 2022, 42(8): 2423

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    Paper Information

    Category: Orginal Article

    Received: Jun. 25, 2021

    Accepted: --

    Published Online: Mar. 17, 2025

    The Author Email: QIAN Cheng (316353009@qq.com)

    DOI:10.3964/j.issn.1000-0593(2022)08-2423-09

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