Spectroscopy and Spectral Analysis, Volume. 42, Issue 8, 2423(2022)

Detection of Melamine and Cyromazine in Raw Milk by Aptamer-Facilitated Gold Nanoparticle-Based Logic Gates

Cheng QIAN1、*, Bo WANG1、1;, Xue-lian FEI1、1;, Pan-cheng YIN1、1;, Bo-tao HUANG2、2;, Hai-bo XING1、1; *;, and Xiao-jun HU1、1; *;
Author Affiliations
  • 11. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China
  • 22. Shanghai Academy of Environmental Sciences, Shanghai 200233, China
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    In this paper, we developed a simple design and a detection method for AND logic gates by using aptamers, Cetyltrimethyl Ammonium Bromide (CTAB), and melamine and cyromazine to control the aggregation and dispersion of gold nanoparticles (AuNPs). First, Based on the fact that aptamer T31 can specifically bind with melamine, and CTAB immediately resulted in the aggregation of AuNPs, an AND gate was fabricated to find whether there was melamine. It also has a detection limit of 0.24 mg·L-1 by the naked eye to detect melamine, and the limit of detection (LOD) by a spectrophotometer is 85 μg·L-1. Second, with the adsorption of aptamer Tcy1 on AuNPs and the strong coordination of Tcy1 with cyromazine, the addition of cyromazine and CTAB immediately resulted in the aggregation of AuNPs, giving rise to another AND gate. It also has a detection limit of 0.17 mg·L-1 by the naked eye, and the limit of detection (LOD) is 9.0 μg·L-1 by spectrophotometer. So, these logic gates can be used to detect melamine and cyromazine in raw milk.

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    Cheng QIAN, Bo WANG, Xue-lian FEI, Pan-cheng YIN, Bo-tao HUANG, Hai-bo XING, Xiao-jun HU. Detection of Melamine and Cyromazine in Raw Milk by Aptamer-Facilitated Gold Nanoparticle-Based Logic Gates[J]. Spectroscopy and Spectral Analysis, 2022, 42(8): 2423

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    Paper Information

    Category: Orginal Article

    Received: Jun. 25, 2021

    Accepted: --

    Published Online: Mar. 17, 2025

    The Author Email: QIAN Cheng (316353009@qq.com)

    DOI:10.3964/j.issn.1000-0593(2022)08-2423-09

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