Laser & Optoelectronics Progress, Volume. 56, Issue 24, 240002(2019)
Imaging Principles and Applications of Super-Resolution Optical Microscopy
Fig. 1. Principle of stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), and fluorescence photoactivation localization microscopy (FPALM)[13]. (a) Target structure; (b) localization of activated probes; (c) super-resolution image
Fig. 2. Basic principle of STED microscopy[15]. (A is simplified Jablonski diagram describing the energy transitions of green fluorescent protein (GFP) during a fluorescence cycle;B is spatial restriction of fluorescence emission using doughnut shaped STED PSF; C is design of a home-built STED microscope for performing two-color super-resolution imaging of green and yellow emitting probes in living brain slices)
Fig. 3. Principle of SIM [22]. (a) Raw image; (b) reconstructed image
Fig. 4. Principle of resolution enhancement of SIM[24]. (a) Sample with detail features; (b) structured illumination pattern; (c) Moire' fringes formed by superposing structured illumination pattern in
Fig. 5. STORM super-resolution reconstruction images of Hela cells[92]. (a) Wide-field fluorescence image; (b) reconstruction image; (c) locally enlarged image
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Yun Fu, Tianle Wang, Sen Zhao. Imaging Principles and Applications of Super-Resolution Optical Microscopy[J]. Laser & Optoelectronics Progress, 2019, 56(24): 240002
Category: Reviews
Received: Apr. 15, 2019
Accepted: Jun. 5, 2019
Published Online: Nov. 26, 2019
The Author Email: Fu Yun (linda_fy@cust.edu.cn)