Spectroscopy and Spectral Analysis, Volume. 40, Issue 11, 3483(2020)
Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry
Figures & Tables(8)
Fig. 1. Chemical structure of three kinds of sulfur-containing amino acids
Fig. 2. Fluorescence emission spectra of different concentrations of SACS, SAC, SAMC and BSA
SACS and SAC (a—g) were (0.00, 3.31, 6.57, 9.80, 13.00, 16.10, 19.20)×10-4 mol·L-1;SAMC(a—g) was half of the previous one (298 K on the A and 310 K on the B group)
Fig. 3. Stern-volmer function of three kinds of sulfur-containing amino acids at different temperature
(a): 298 K; (b): 310 K
Fig. 4. Stern-Volmer function of three kinds of sulfur-containing amino acid at different temperature
Fig. 5. Spectrum overlap of UV-Visible absorption spectrum and fluorescence of SAMC and emission spectrum of BSA
Table 1. Linear equations, correlation coefficients and fluorescence quenching constants of three sulfur-containing amino acids and BSA
View tableView in ArticleTable 1. Linear equations, correlation coefficients and fluorescence quenching constants of three sulfur-containing amino acids and BSA
化合物种类 KSV/
(L·mol-1)Kq/[L·(mol·s)-1] Stern-Volmer
equationR2 T/K SACS 3.5 3.5×108 y=0.003 5x+1.006 7 0.999 4 298 3.7 3.7×108 y=0.003 7x+1.000 9 0.984 2 310 SAC 7.1 7.1×108 y=0.007 1x+0.988 2 0.990 8 298 7.0 7.0×108 y=0.007 0x+1.012 7 0.992 1 310 SAMC 1.34×103 1.34×1011 y=0.134 0x+0.932 7 0.988 4 298 7.16×102 7.16×1010 y=0.071 6x+0.963 9 0.991 7 310
Table 2. SAMC and BSA binding constants and number of binding sites
View tableView in ArticleTable 2. SAMC and BSA binding constants and number of binding sites
化合物 n lgKA KA/
(L·mol-1)R2 T/K SAMC 1.224 0 3.791 1 6.18×103 0.998 6 298 1.294 2 3.743 2 5.54×103 0.998 5 310
Table 3. Thermodynamic parameters of SAMC at different temperatures
View tableView in ArticleTable 3. Thermodynamic parameters of SAMC at different temperatures
化合物 ΔH/
(kJ·mol-1)ΔG/
(kJ·mol-1)ΔS/
(J·mol-1·K-1)T/
KSAMC -7.06 -21.63 48.89 298 -22.21 46.99 310
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Xing-xing LI, Xiang ZHANG, Xue-song HUANG. Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3483
Paper Information
Category: Research Articles
Received: Jul. 6, 2019
Accepted: --
Published Online: Jun. 18, 2021
The Author Email: LI Xing-xing (931244501@qq.com)
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