Laser & Optoelectronics Progress, Volume. 58, Issue 3, 3000041(2021)

Measurement Method of Optical Property Parameters of Biological Tissue

Chenyang Lü and Zhan Renjun*
Author Affiliations
  • College of Equipment Management and Support, Engineering University of People''s Armed Police, Xi''an , Shaanxi 710086, China
  • show less
    Figures & Tables(10)
    Schematic of experimental setup[21]
    Schematic of DIS system[34]
    Kubelka-Munk theory flux
    Schematic of IAD/IS system[103]
    IAD/IMC system logic diagram[120]
    Relationship of optical parameter measurement methods of biological tissue
    • Table 1. Classification and feature comparison of main measurement techniques

      View table

      Table 1. Classification and feature comparison of main measurement techniques

      ClassificationAdvantageLimitation
      SIS

      ① No need to measure reflectivity of inner wall

      ② Two integrating spheres have no influence on each other

      ③ Calibration of system is easier and experimental device is simpler

      ④ Lower cost

      ① Low measurement accuracy

      ② Can only be measured in steps

      ③ Sample cannot be illuminated diffusely in transmission geometry

      DIS

      ① Most accurate method at present

      ② Suitable for layered structure organization

      ③ Diffuse reflectivity and diffuse transmittance can be measured at same time

      ④ Sample can be illuminated by diffuse reflection in transmission or reflection geometry

      ① Demand for stability of light source is very high

      ② It is difficult to implement and calibrate

      ③ Price is relatively high

      PSM① Measurement of absorption coefficient in Ultraviolet Band

      ① It is only suitable for in vitro measurement

      ② Scope of application is narrow

      CW① Information of organizational structure and function can be obtained at same time① Only large tissues and organs can be measured
      ② Limited to in vivo measurement
      Micro-area

      ① Measurement accuracy is higher

      ② Micro-areas can be measured

      ① Theory of photon transmission in micro-area is not accurate enough
      ② Limited to in vivo measurement
    • Table 2. Comparative analysis of different measurement methods

      View table

      Table 2. Comparative analysis of different measurement methods

      ClassificationAdvantageLimitation
      Direct measurement①Analytical expression is very simple

      ①Experimental conditions specified in model must be strictly met

      ②Error is large for plane medium with multiple scattering

      Indirect measurement

      ①Can use computer-aided means

      ②High flexibility and accuracy

      ①Analytical method is complicated
    • Table 3. Applicable conditions and comparative analysis of indirect measurement method

      View table

      Table 3. Applicable conditions and comparative analysis of indirect measurement method

      ClassificationApplicable conditionAdvantageLimitation
      Non-iterative method

      ① Scattering is obviously stronger than absorption

      ② Relationship between optical parameters and measured parameters is an implicit function

      ① Mathematical form is simple

      ① It only aims at one-dimensional diffuse radiation that scattering far exceeds absorption.

      ② Theoretical model is not perfect

      Iterative method

      ① Samples with differences in refractive index at boundary

      ② Samples with multi-layer properties

      ① Theoretical model is more perfect

      ② Non-destructive measurement

      ① Using complex solution of transport equation
    • Table 4. Application scope and comparative analysis of different iterative measurement methods

      View table

      Table 4. Application scope and comparative analysis of different iterative measurement methods

      ClassificationApplicable scopeAdvantageLimitation
      IADSamples with prominent anisotropic scattering and internal boundary reflection

      ① Iterative solution can be obtained quickly with help of computer

      ② Anisotropic scattering and internal reflection at boundary can be flexibly included

      ③Result is very accurate when illumination diameter is less than sample diameter

      Solution can only be solved under conditions that tissue thickness is limited, optical parameters are uniformly distributed and solution process is independent of time
      IMCIt is most effective for tissues based on real shapes or anatomical structures

      ① Recognized as most accurate measurement method at present

      ② Loss of light at edge of sample can be accurately calculated

      Amount of calculation is large and calculation time is long
    Tools

    Get Citation

    Copy Citation Text

    Chenyang Lü, Zhan Renjun. Measurement Method of Optical Property Parameters of Biological Tissue[J]. Laser & Optoelectronics Progress, 2021, 58(3): 3000041

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Reviews

    Received: Jun. 5, 2020

    Accepted: --

    Published Online: Mar. 12, 2021

    The Author Email: Renjun Zhan (zhanrenjun@aliyun.com)

    DOI:10.3788/LOP202158.0300004

    Topics