Raman Spectroscopic Characteristic Structure Analysis and Rapid Identification of Food-Borne Pathogen Spores Based on SERS Technology

Shi-jie LIU; Yao-di ZHU; Miao-yun LI; Gai-ming ZHAO; Li-jun ZHAO; Yang-yang MA; Na WANG

doi:10.3964/j.issn.1000-0593(2022)09-2774-07

Spectroscopy and Spectral Analysis, Volume. 42, Issue 9, 2774(2022)

Raman Spectroscopic Characteristic Structure Analysis and Rapid Identification of Food-Borne Pathogen Spores Based on SERS Technology

Shi-jie LIU*... Yao-di ZHU1; 2;, Miao-yun LI1; 2; *;, Gai-ming ZHAO1; 2;, Li-jun ZHAO1; 2;, Yang-yang MA1; 2; and Na WANG1; |Show fewer author(s)

Author Affiliations

1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450000, China

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Figures & Tables(9)

Fig. 1. Phase-contrast microscopic image of three food-borne pathogenic bacteria spores

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Fig. 2. UV-Vis absorption spectrum (a), particle size distribution (b) and TEM image (c) of AgNPs

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Fig. 3. SERS enhancement effect of AgNPs on three food-borne pathogenic bacteria spores

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Fig. 4. SERS average fingerprints of three food-borne pathogenic bacteria spores

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Fig. 5. Repeatability test of SERS spectra of three food-borne pathogenic bacteria spores

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Fig. 6. Two-dimensional scatter plot of principal component analysis (PCA) of SERS maps of spores of three food-borne pathogens

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Fig. 7. HCA resulting dendrogram of SERS maps of spores of three food-borne pathogens

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Table 1. Tentative attribution of Raman shift of SERS spectra of three food-borne pathogenic bacteria spores

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Table 1. Tentative attribution of Raman shift of SERS spectra of three food-borne pathogenic bacteria spores

拉曼位移/cm^-1 (本研究)	拉曼位移/cm^-1 (文献资料)	峰位归属	C. perfringens spores	C. difficile spores	B. cereus spores
425~434	481	碳水化合物的骨架模式(淀粉)	+	+	+
553~559	540~560	S—S拉伸(蛋白质)	++	++	++
657~663	662	Ca²⁺-DPA	+	++	+
774~778	776	腺嘧啶或尿嘧啶(核酸)	++	++	++
818~820	810~827	Ca²⁺-DPA	+	+	+
890	880~900	=C—O—C=拉伸	-	+	-
936	930~957	磷脂N—C拉伸	+	-	-
1 017	1 017	Ca²⁺-DPA	++++	++++	++++
1 091~1 098	1092	O=P—O伸缩振动(核酸)	++	++	+++
1 233~1 236	1 220~1 248	C—H₂拉伸振动	+	+	+
1 271	1 273	酰胺Ⅲ(蛋白质)	+	-	-
1 294	1 297	CH₂变形振动(脂质)	++	-	-
1 327~1 331	1 323~1 334	C—H变形振动(蛋白质)	+	+	+
1 389~1 393	1 393, 1 397	Ca²⁺-DPA	++	++	+
1 441~1 449	1 445, 1 448	Ca²⁺-DPA	++	++	++
1 572~1 576	1 570~1 576	Ca²⁺-DPA	+++	+++	+++
1 609	1 616	酪氨酸(蛋白质)	+	-	-
1 649	1 655	酰胺Ⅰ(蛋白质)	++	-	-
1 661~1 669	1 665	酰胺Ⅰ(不饱和脂质)	++	++	++
1 768~1 772	1 745	酯的C=O基团伸缩	+	+	-

Table 2. Comparison of six characteristic peaks of Ca²⁺-DPA in SERS spectra of three spores

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Table 2. Comparison of six characteristic peaks of Ca²⁺-DPA in SERS spectra of three spores

特征峰编号	C. perfringens spores	C. difficile spores	B. cereus spores
C₁	691.56±111.44^b	3 203.56±114.32^a	514.07±164.20^c
C₂	1 627.82±110.73^b	2 961.78±116.63^a	-
C₃	12 848.73±122.95^b	16 867.77±129.82^a	11 035.27±174.48^c
C₄	2 364.93±135.98^b	3 536.54±141.93^a	760.33±200.46^c
C₅	5 252.11±137.85^b	7 083.81±145.53^a	4 860.83±201.51^c
C₆	8 317.11±136.46^b	8 849.84±151.83^a	6 924.89±197.80^c

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Shi-jie LIU, Yao-di ZHU, Miao-yun LI, Gai-ming ZHAO, Li-jun ZHAO, Yang-yang MA, Na WANG. Raman Spectroscopic Characteristic Structure Analysis and Rapid Identification of Food-Borne Pathogen Spores Based on SERS Technology[J]. Spectroscopy and Spectral Analysis, 2022, 42(9): 2774

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Paper Information

Category: Research Articles

Received: Jul. 17, 2021

Accepted: Nov. 17, 2021

Published Online: Nov. 17, 2022

The Author Email: LIU Shi-jie (15938978937@163.com)

DOI:10.3964/j.issn.1000-0593(2022)09-2774-07

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laser devices and laser physics

Lasers and Laser Optics

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Instrumentation, Measurement and Metrology

Table 1. Tentative attribution of Raman shift of SERS spectra of three food-borne pathogenic bacteria spores

Table 1. Tentative attribution of Raman shift of SERS spectra of three food-borne pathogenic bacteria spores

Table 2. Comparison of six characteristic peaks of Ca2+-DPA in SERS spectra of three spores

Table 2. Comparison of six characteristic peaks of Ca2+-DPA in SERS spectra of three spores

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Table 2. Comparison of six characteristic peaks of Ca²⁺-DPA in SERS spectra of three spores

Table 2. Comparison of six characteristic peaks of Ca²⁺-DPA in SERS spectra of three spores