Laser & Optoelectronics Progress, Volume. 58, Issue 18, 1811009(2021)
Progress in Computational Fourier Microscopy
Fig. 1. Schematic diagram of a conventional Fourier microscope
Fig. 2. Light-path diagram of a transmission computational Fourier microscope[27]. (a) Light wave is emitted from on-axis point; (b) light wave is emitted from off-axis point; (c) light waves are simultaneously emitted from all point sources on the LED
Fig. 3. Experimental setup diagram of transmission computational Fourier microscope [27]
Fig. 4. Brightfield microscopic imaging results of onion epidermal cells [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b3) circular digital masks with different radii; (c1)--(c3) computationally detected circular Fourier spectra; (d1)--(d3) reconstructed brightfield images using computationally detected circular Fourier spectra
Fig. 5. Darkfield microscopic imaging results of onion epidermal cells [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b4) annular digital masks with different radii; (c1)--(c4) computationally detected annular Fourier spectra; (d1)--(d4) reconstructed darkfield images using computationally detected annular Fourier spectra
Fig. 6. Semicircular DPC imaging results of onion epidermal cells from different orientations[27]. (a) Fourier spectrum image captured by the camera; (b1)--(b8) semicircular digital masks with different orientations; (c1)--(c8) computationally detected semicircular Fourier spectra; (d1)--(d4) reconstructed DPC images using computationally detected semicircular Fourier spectra
Fig. 7. Semicircular DPC imaging results of onion epidermal cells from different radii[27]. (a) Fourier spectrum image captured by the camera; (b1)--(b6) semicircular digital masks with different radii; (c1)--(c6) computationally detected semicircular Fourier spectra; (d1)--(d3) reconstructed DPC images using computationally detected semicircular Fourier spectra
Fig. 8. Semi-annular DPC imaging results of onion epidermal cells from different orientations [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b8) semi-annular digital masks with different orientations; (c1)--(c8) computationally detected semi-annular Fourier spectra; (d1)--(d4) reconstructed DPC images using computationally detected semi-annular Fourier spectra
Fig. 9. Semi-annular DPC imaging results of onion epidermal cells from different radii [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b6) semi-annular digital masks with different radii; (c1)--(c6) computationally detected semi-annular Fourier spectra; (d1)--(d3) reconstructed DPC images using computationally detected semi-annular Fourier spectra
Fig. 10. Schematic diagram of computational Fourier microscope achieving angular sampling. (a) Specimen in the focal plane of objective lens; (b)(c) specimen in the de-focal plane of objective lens
Fig. 11. Perspective images recovered from different locations of single-pixel detectors. (a1)--(a4) Rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) perspective images retrieved from the rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
Fig. 12. Digitally refocused images. (a) z=-77 μm; (b) z=-10 μm; (c) z=37 μm
Fig. 13. Light-path diagram of reflection computational Fourier microscope [34]
Fig. 14. Perspective images recovered from different locations of single-pixel detectors[34]. (a1)--(a4) Perspective images retrieved from bottommost, rightmost, topmost, and leftmost single-pixel detectors, respectively; (b1)--(b4) bottommost, rightmost, topmost, and leftmost single-pixel detectors, respectively
Fig. 15. Digitally refocused images of reflective specimen [34].(a) z=-15 μm; (b) z=10 μm; (c) z=32 μm
Fig. 16. Color image is reconstructed based on the scheme of sampling the color information of the specimen in the detection side [35]. (a1)--(a3) Three-channel light intensity signal separated from the image captured by the camera; (b1)--(b3) sample image corresponding to the three channels reconstructed from the three-channel light intensity signal; (c) color-perspective image synthesized by
Fig. 17. Perspective images recovered from different locations of single-pixel detectors [35]. (a1)--(a4) Perspective images retrieved from rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
Fig. 18. Digitally refocused color images[35].(a) z=-77 μm; (b) z=-10 μm; (c) z=37 μm
Fig. 19. Principle of generating color-coded structured light patterns[36]
Fig. 20. Color images reconstructed based on the scheme of sampling the color information of the specimen at the illumination side [35]. (a) 1D light intensity sequence recorded by a single-pixel detector; (b) image of specimen recovered from the 1D light intensity sequence; (c) partially enlarged view of
Fig. 21. Perspective images recovered from different locations of single-pixel detectors [35]. (a1)--(a4) Rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) perspective images retrieved from rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
Fig. 22. Digitally refocused color images[35]. (a) z=-53 μm; (b) z=22 μm; (c) z=-59 μm
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Jingang Zhong, Manhong Yao, Junzheng Peng. Progress in Computational Fourier Microscopy[J]. Laser & Optoelectronics Progress, 2021, 58(18): 1811009
Category: Imaging Systems
Received: Jun. 23, 2021
Accepted: Aug. 11, 2021
Published Online: Sep. 3, 2021
The Author Email: Zhong Jingang (tzjg@jnu.edu.cn)