Laser & Optoelectronics Progress, Volume. 58, Issue 18, 1811009(2021)

Progress in Computational Fourier Microscopy

Jingang Zhong1、*, Manhong Yao2, and Junzheng Peng1
Author Affiliations
  • 1Department of Optoelectronic Engineering, Jinan University, Guangzhou, Guangdong 510632, China
  • 2School of Optoelectronic Engineering, Guangdong Polytechnic Normal University, Guangzhou, Guangdong 510665, China
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    Figures & Tables(22)
    Schematic diagram of a conventional Fourier microscope
    Light-path diagram of a transmission computational Fourier microscope[27]. (a) Light wave is emitted from on-axis point; (b) light wave is emitted from off-axis point; (c) light waves are simultaneously emitted from all point sources on the LED
    Experimental setup diagram of transmission computational Fourier microscope [27]
    Brightfield microscopic imaging results of onion epidermal cells [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b3) circular digital masks with different radii; (c1)--(c3) computationally detected circular Fourier spectra; (d1)--(d3) reconstructed brightfield images using computationally detected circular Fourier spectra
    Darkfield microscopic imaging results of onion epidermal cells [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b4) annular digital masks with different radii; (c1)--(c4) computationally detected annular Fourier spectra; (d1)--(d4) reconstructed darkfield images using computationally detected annular Fourier spectra
    Semicircular DPC imaging results of onion epidermal cells from different orientations[27]. (a) Fourier spectrum image captured by the camera; (b1)--(b8) semicircular digital masks with different orientations; (c1)--(c8) computationally detected semicircular Fourier spectra; (d1)--(d4) reconstructed DPC images using computationally detected semicircular Fourier spectra
    Semicircular DPC imaging results of onion epidermal cells from different radii[27]. (a) Fourier spectrum image captured by the camera; (b1)--(b6) semicircular digital masks with different radii; (c1)--(c6) computationally detected semicircular Fourier spectra; (d1)--(d3) reconstructed DPC images using computationally detected semicircular Fourier spectra
    Semi-annular DPC imaging results of onion epidermal cells from different orientations [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b8) semi-annular digital masks with different orientations; (c1)--(c8) computationally detected semi-annular Fourier spectra; (d1)--(d4) reconstructed DPC images using computationally detected semi-annular Fourier spectra
    Semi-annular DPC imaging results of onion epidermal cells from different radii [27]. (a) Fourier spectrum image captured by the camera; (b1)--(b6) semi-annular digital masks with different radii; (c1)--(c6) computationally detected semi-annular Fourier spectra; (d1)--(d3) reconstructed DPC images using computationally detected semi-annular Fourier spectra
    Schematic diagram of computational Fourier microscope achieving angular sampling. (a) Specimen in the focal plane of objective lens; (b)(c) specimen in the de-focal plane of objective lens
    Perspective images recovered from different locations of single-pixel detectors. (a1)--(a4) Rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) perspective images retrieved from the rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
    Digitally refocused images. (a) z=-77 μm; (b) z=-10 μm; (c) z=37 μm
    Light-path diagram of reflection computational Fourier microscope [34]
    Perspective images recovered from different locations of single-pixel detectors[34]. (a1)--(a4) Perspective images retrieved from bottommost, rightmost, topmost, and leftmost single-pixel detectors, respectively; (b1)--(b4) bottommost, rightmost, topmost, and leftmost single-pixel detectors, respectively
    Digitally refocused images of reflective specimen [34].(a) z=-15 μm; (b) z=10 μm; (c) z=32 μm
    Color image is reconstructed based on the scheme of sampling the color information of the specimen in the detection side [35]. (a1)--(a3) Three-channel light intensity signal separated from the image captured by the camera; (b1)--(b3) sample image corresponding to the three channels reconstructed from the three-channel light intensity signal; (c) color-perspective image synthesized by Fig. 16 (b1)--(b3)
    Perspective images recovered from different locations of single-pixel detectors [35]. (a1)--(a4) Perspective images retrieved from rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
    Digitally refocused color images[35].(a) z=-77 μm; (b) z=-10 μm; (c) z=37 μm
    Principle of generating color-coded structured light patterns[36]
    Color images reconstructed based on the scheme of sampling the color information of the specimen at the illumination side [35]. (a) 1D light intensity sequence recorded by a single-pixel detector; (b) image of specimen recovered from the 1D light intensity sequence; (c) partially enlarged view of Fig. 20(b); (d) color image recovered by demosaicing from Fig. 20 (b)
    Perspective images recovered from different locations of single-pixel detectors [35]. (a1)--(a4) Rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively; (b1)--(b4) perspective images retrieved from rightmost, topmost, leftmost, and bottommost single-pixel detectors, respectively
    Digitally refocused color images[35]. (a) z=-53 μm; (b) z=22 μm; (c) z=-59 μm
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    Jingang Zhong, Manhong Yao, Junzheng Peng. Progress in Computational Fourier Microscopy[J]. Laser & Optoelectronics Progress, 2021, 58(18): 1811009

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    Paper Information

    Category: Imaging Systems

    Received: Jun. 23, 2021

    Accepted: Aug. 11, 2021

    Published Online: Sep. 3, 2021

    The Author Email: Zhong Jingang (tzjg@jnu.edu.cn)

    DOI:10.3788/LOP202158.1811009

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