Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618009(2024)
Noncoherent Raman Spectroscopy and Its Biomedical Application (Invited)
Fig. 1. (a) Schematic diagram of energy level structures for Rayleigh scattering and Raman scattering processes; (b) schematic diagram of Rayleigh scattering and Raman scattering spectra
Fig. 2. (a) Schematic diagram of a handheld Raman spectroscopy detection system; (b) schematic diagram of confocal Raman microscopy system
Fig. 3. Localized surface plasmon resonance[13]
Fig. 4. Electric field enhancement mechanism of SERS[13]
Fig. 5. Chemical enhancement mechanism of SERS, modified from reference [16]. (a) The non resonant enhancement between the tested molecule and metal nanoparticles is independent of excitation; (b) resonance enhancement formed by laser energy and electronic transitions within the tested molecule; (c) class resonance enhancement of photo induced charge transfer
Fig. 7. General process of Raman spectroscopy data processing
Fig. 8. Workflow of machine learning[54]
Fig. 10. The application of incoherent Raman microscopy spectroscopy technology at the cellular level. (a) H&E staining maps, protein and lipid distribution maps, as well as spontaneous Raman spectra and classical least squares fitting of normal colon tissue (upper) and cancerous colon tissue (lower)[63]; (b) SERS immunoassay principle diagram for epithelial mesenchymal transition[64]; (c) a blood biochemical map drawn at the cellular level using spontaneous Raman microscopy spectroscopy[65]; (d) mapping metabolic changes in endothelial cells using spontaneous Raman probes[66]
Fig. 12. The application of Raman microspectral technology in body fluid biopsy. (a) Schematic diagram of SERS technology combined with artificial intelligence using exosomes in plasma to detect various cancers[89]; (b) schematic diagram of the preparation process of saliva protein silver nanoparticles mixture, and comparison of SERS spectra of the mixture with saliva protein and silver nanoparticles without silver[90]
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Yuyi Li, Yue Gan, Ben Niu, Jing Huang, Qiuqiang Zhan. Noncoherent Raman Spectroscopy and Its Biomedical Application (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618009
Category: Microscopy
Received: Feb. 5, 2024
Accepted: Feb. 19, 2024
Published Online: Mar. 18, 2024
The Author Email: Huang Jing (jing_huang@scnu.edu.cn), Zhan Qiuqiang (zhanqiuqiang@m.scnu.edu.cn)