Journal of Radiation Research and Radiation Processing, Volume. 42, Issue 2, 020401(2024)
Molecular characteristics of rice chimeras containing gene mutations induced by carbon ion beam radiation
Fig. 1. SNPs density distribution of rice 10 kb (10 325 SNPs) liquid chip (color online)
Fig. 3. Analysis of OsNramp5 mutations in rice: (a) fan-shaped mutated pattern of rice chimeric line with OsNramp5 gene mutation; (b) heterozygous mutant sequencing result of rice panicle; (c) wild-type sequencing result of rice panicle (color online)
Fig. 4. Genome sequencing variation locus analysis in rice M1 chimeric mutant: (a) the proportion of SNP variation in different structural domains of whole M1 genome; (b) the variant loci number of SNP substitution types on whole M1 genome (color online)
Fig. 5. Differential sites located on genome between chimeric mutant and CK: (a) the variation sites of panicle with OsNramp5 mutation; (b) the variation sites of panicle without OsNramp5 mutation (color online)
Fig. 6. Osnramp5 genotype: (a) the Osnramp5 genotype of the original heterozygous rice stump and 284 per plant in M2 generation; (b) regenerated tillers oforiginal heterozygous rice stump (color online)
Fig. 7. Variation types in M2 generation of chimeric mutantwith OsNramp5 mutation; (a) the number of heterozygous and homozygous SNP/InDel variant locus in M2 generation; (b) the variant loci number of different SNP substitution types in M2 generation (color online)
Fig. 8. Variation types and its inheritance of whole-genome in M1 generation: (a) the substitution types and variant loci number in M-92 panicle with OsNramp5mutation; (b) genetic variation pattern and SNP number of M-92 genomic variation (color online)
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Siwei LYU, Li ZHANG, Yiyan ZHANG, Yuanhai ZHANG, Xuanming PENG, Zhen YANG. Molecular characteristics of rice chimeras containing gene mutations induced by carbon ion beam radiation[J]. Journal of Radiation Research and Radiation Processing, 2024, 42(2): 020401
Category: Research Articles
Received: Dec. 22, 2023
Accepted: Feb. 22, 2024
Published Online: May. 28, 2024
The Author Email: YANG Zhen (杨震)