Journal of Radiation Research and Radiation Processing, Volume. 42, Issue 2, 020401(2024)

Molecular characteristics of rice chimeras containing gene mutations induced by carbon ion beam radiation

Siwei LYU... Li ZHANG, Yiyan ZHANG, Yuanhai ZHANG, Xuanming PENG and Zhen YANG* |Show fewer author(s)
Author Affiliations
  • Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Changsha 410125, China
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    Figures & Tables(11)
    SNPs density distribution of rice 10 kb (10 325 SNPs) liquid chip (color online)
    Molecular probe sequence of the OsNramp5 gene
    Analysis of OsNramp5 mutations in rice: (a) fan-shaped mutated pattern of rice chimeric line with OsNramp5 gene mutation; (b) heterozygous mutant sequencing result of rice panicle; (c) wild-type sequencing result of rice panicle (color online)
    Genome sequencing variation locus analysis in rice M1 chimeric mutant: (a) the proportion of SNP variation in different structural domains of whole M1 genome; (b) the variant loci number of SNP substitution types on whole M1 genome (color online)
    Differential sites located on genome between chimeric mutant and CK: (a) the variation sites of panicle with OsNramp5 mutation; (b) the variation sites of panicle without OsNramp5 mutation (color online)
    Osnramp5 genotype: (a) the Osnramp5 genotype of the original heterozygous rice stump and 284 per plant in M2 generation; (b) regenerated tillers oforiginal heterozygous rice stump (color online)
    Variation types in M2 generation of chimeric mutantwith OsNramp5 mutation; (a) the number of heterozygous and homozygous SNP/InDel variant locus in M2 generation; (b) the variant loci number of different SNP substitution types in M2 generation (color online)
    Variation types and its inheritance of whole-genome in M1 generation: (a) the substitution types and variant loci number in M-92 panicle with OsNramp5mutation; (b) genetic variation pattern and SNP number of M-92 genomic variation (color online)
    • Table 1. Agronomic traits of chimeric line with OsNramp5 gene mutation

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      Table 1. Agronomic traits of chimeric line with OsNramp5 gene mutation

      穗编号

      Panicle No.

      穗长

      Panicle length

      实粒数

      Full-grain count

      空壳数

      Unfilled-grain count

      总粒数

      Total grain count

      结实率 / %

      Seed-set rate

      M-9228.814217131345.37
      M-126.711314926243.13
      M-221.4657413946.76
      M-421.4567813441.79
      W-9325.312921434337.61
      W-325.010211421647.22
      W-525.810712022747.13
      W-630.510128438526.23

      平均

      Mean

      25.610215125340.37
    • Table 2. SNP allele variation among OsNramp5 chimeric panicle mutants in M1 population

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      Table 2. SNP allele variation among OsNramp5 chimeric panicle mutants in M1 population

      样品名称

      Sample name

      编号

      Code

      与CK相似度 / %

      Similarity percentage

      to CK

      相似性分析Similarity analysis

      SBS突变数(外显子区)

      Single-base-substitution mutation count (SBS in exon region)

      InDel突变位点

      InDel mutation loci

      Nramp5基因型

      Nramp5genotype

      92-1M-199.7325(2)1杂合型Heterozygous
      92-2M-299.7522(3)2杂合型Heterozygous
      92-4M-499.7127(1)1杂合型Heterozygous
      92M-9299.7424(0)1杂合型Heterozygous
      92-3W-399.7424(3)1野生型Wild type
      92-5W-599.7721(1)1野生型Wild type
      92-6W-699.7226(2)1野生型Wild type
      93W-9399.6534(5)0野生型Wild type
    • Table 3. SNPs variation rate of OsNramp5 chimeric mutant in M2 generation

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      Table 3. SNPs variation rate of OsNramp5 chimeric mutant in M2 generation

      M2编号

      M2 code

      与M1相似度 / %

      Similarity percentage to M1

      与M1差异位点

      Number of differential sites with M1

      变异位点中M1占比 / %

      Percentage of M1 in variant loci

      M2-199.208012.5
      M2-299.188214.6
      M2-399.208011.3
      M2-499.188213.4
      M2-599.217913.9
      M2-699.247613.2
      M2-799.217913.9
      M2-899.237714.3
      M2-999.168410.7
      M2-1099.198113.6
      M2-1199.247611.8
      M212410.5
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    Siwei LYU, Li ZHANG, Yiyan ZHANG, Yuanhai ZHANG, Xuanming PENG, Zhen YANG. Molecular characteristics of rice chimeras containing gene mutations induced by carbon ion beam radiation[J]. Journal of Radiation Research and Radiation Processing, 2024, 42(2): 020401

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    Paper Information

    Category: Research Articles

    Received: Dec. 22, 2023

    Accepted: Feb. 22, 2024

    Published Online: May. 28, 2024

    The Author Email: YANG Zhen (杨震)

    DOI:10.11889/j.1000-3436.2023-0117

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