The emission properties of 2 \mu m region fluorescence of Tm^³⁺-Ho^³⁺ co-doped tellurite glasses are investigated. Introducing F^- ions to the composition of tellurite glasses plays a positive effect on the 2 \mu m emission. A maximum intensity of 2 \mu m emission is achieved when 1.5-mol% Tm₂O₃ and 1-mol% Ho₂O₃ concentration are doped in the glasses. The emission cross section and gain coefficient of the ⁵I₈-⁵I₇ transition of Ho^³⁺ are calculated. The emission cross section has a maximum of 1.29×10^-20 cm² at 2048 nm wavelength. The results indicate that Tm^³⁺-Ho^³⁺ co-doped tellurite glasses are suitable for 2 \mu m application.