As deposited 18Ni300 was prepared by selective laser melting, the “ball-on-disk” sliding wear test were performed to study its wear characteristics with different sliding speed and load. Friction factor were recorded during the test, and the wear tracks were analyzed by Contour GT-K 3D profilometer to calculate the volume wear rate. The microstructure and wear area were analyzed by HITACHI SU3500 SEM, and the chemical composition of partial wear area were analyzed by EDS. The results show that: friction factor increased with the increase of sliding, and decreased with the increase of testing load. The volume wear rate increased with the increase of sliding speed and testing load. When the sliding speed was 0.4 m/s, the wear mechanism were oxidation wear and abrasive wear under the testing load of 15 N and 20 N, the wear mechanism turned to adhesive wear and abrasive wear with the increasing of the sliding speed. The wear mechanism was abrasive wear and oxidation wear under the 1.2 m/s sliding speed and 20 N testing load.