The heart development genes of Drosophila melanogaster are highly conserved with human heart development genes. Lbe (Ladybird early) is one of the important genes involved in Drosophila heart development. In order to further understand the regulatory role of Lbe gene in heart development, the anti-Lbe antibody is prepared with DNA immunization technique. The Lbe coding sequence was amplified with PCR, and then it was homologously recombined with the eukaryotic expression vector pCAGGS-P7. This homologous recombinant vector was used as antigen to immunize four weeks old mice to obtain the serum containing anti-Lbe polyclonal antibody. Western blot and embryo antibody staining results showed that the specificity of the antibody was good. The antibody provides a foundation for the subsequent study of function of Lbe.