A micro-arrayed multichannel optical filter is proposed to allow the colposcopy to have an ability of multispectral imaging. The micro-arrayed filter is fabricated by micro-lithographic procedures and vacuum multilayer thin film coatings, and the wavelengths of light passing through the filter correspond to the characteristic peaks of the biomarkers in the reflectance or fluorescence spectra. After integrating the filter with an image sensor on colposcopy, multiple spectral images centered at different wavelengths can be obtained to provide the information about morphological changes on cervical surface and the quantitative variation of the biomarkers in cervical tissues. The micro-arrayed filter with four kinds of filter cells (λ1=630 nm, λ2=460 nm, λ3=515 nm, λ4=577 nm) has been fabricated successfully with an area of 10 μm×10 μm for each filter cell. The bandwidth of each filter cell is about 40 nm, and the transmittance is between 30% and 40%. Experiments demonstrate that the optical properties of micro-arrayed filter satisfy the basic requirements of multispectral imaging. After integrating with a colposcope, the sensitivity and specificity of colposcope diagnosis is improved significantly, which can reduce the biopsy frequencies for patients.