We establish an accurate and prompt intraoperative sentinel lymph node (SLN) diagnostic system using in laparoscopy for gastric cancer. The system consists of a near infrared (NIR) laser, a near infrared camera, a spectroscope, two optical fibers, a monitor and imaging analyzer. The experiments on animals are performed to assess the availability of this system. It is able to verify fluorescence imaging and spectrum of gastric SLN, after injecting indocyanine green (ICG) (1 ml: 0.5?1 mg/ml) to the subserosa.