In close range industrial photogrammetry, the automatic focusing of lens will lead to the fluctuation of camera parameters, resulting in the reduction of measurement accuracy. In order to solve this problem, a layered camera calibration method is proposed. The camera calibration technology based on plane checkerboard is analyzed, the automatic focusing principle of digital SLR camera and how it affects the change of camera principal distance are expounded, and the basic idea of the layered camera calibration method is emphatically analyzed. The layered camera calibration experiment reveals the variation law of the camera internal parameters and distortion coefficients with the calibration distances. The result of accuracy evaluation experiment shows that selecting appropriate camera internal parameters according to the photographing distance can significantly improve the accuracy of close range industrial photogrammetry.