A method of stripe gray-scale asymmetry adjustment for strip center extraction is proposed to solve the problem that stripe gray-scale asymmetric distribution degrades the accuracy of stripe center extraction in the structured light measurement. Firstly, the exponentiation of initial gray-scale is obtained. The initial gray-scale is subtracted from exponential calculation result, and the subtraction result is gray-compensated. Then, the gray-scale after compensation is used to iterate until the iteration termination conditions are satisfied. Moreover, the factors that affect the asymmetry of stripe gray-scale distribution are analyzed, and the evaluation parameter of the stripe gray-scale distribution is put forward. Experiments are conducted to verify the proposed method. The results show that the algorithm can effectively adjust the stripe gray-scale according to the initial gray-scale distribution. The iteration result highlights the gray information around the maximum value, reduces the impact of gray-scale asymmetric distribution on stripe center extraction, and improves the accuracy of stripe center extraction. Based on these results, the validity and reliability of this method are verified.