In order to ensure the reliable communication using prefabricated optical fiber connectors in the precision control environment, and to meet the requirements of multi-mode and single-mode optical fibers in terms of transmission performance, a low-loss prefabricated optical fiber connection ferrule with guided positioning was designed. By analyzing the insertion loss, tilt loss and gap loss of the optical fiber ferrule, two design methods were adopted to reduce the ferrule connection loss: the structural design of the ferrule inclination angle and error compensation, and the matching of the end coating material. Precise positioning of ferrule pairing was achieved by aligning the MT sleeve with the PIN guide pin and guide hole. Based on the connection loss of 850 nm and 1 300 nm multi-mode and 1 310 nm and 1 550 nm single-mode optical fibers, tests were conducted by using the BINNA-MTS 3D interferometer. The results show that the average loss of prefabricated ferrule docking is about 0.3 dB and 0.24 dB, the maximum loss added value of mechanical properties and environmental test is 0.19 dB, and the maximum value is much less than 0.8 dB, which indicates that the end face and alignment performance of the fiber connection ferrule meet the requirements.