In order to study the new wavelength output of the alkali metal rubidium vapor laser and its possible applications, the two-photon excitation and four-wave mixing process of 5 2S1/2→6 2D5/2 and 5 2S1/2→6 2D3/2 in the rubidium atomic system produced laser output with four wavelengths of 358.7 nm, 359.1 nm, 420.3 nm, and 421.7 nm, obtained the best resonance position and resonance linewidth of 6 2D5/2 and 6 2D3/2 energy levels, and studied the generated four-wave mixing laser. The signal intensity changes with the temperature of the system and the energy of the single pulse of the pump light. An abnormal transition of 6 2D5/2→6 2P1/2 is found when the system temperature is higher than 185 ℃ and a preliminary explanation is given. Experimental results show that this technology of generating blue-violet lasers through four-wave mixing of rubidium vapor can provide a new laser source for marine resource exploration, high-density information storage, and underwater laser communications.