Super-resolution localization imaging breaks the diffraction limit and enables optical imaging with a spatial resolutionof20 to50nanometers. However，traditionalsuper-resolutionimaging cannotanalysethespectralinformationof thefluorescentmolecules. We developedspectroscopicphotonlocalizationmicroscopy(SPLM)，whichcansimultaneously analyse the spatial and spectral information of single fluorescent molecule. Using SPLM，we demonstrated simultaneous multi-molecular super-resolution imaging，where the number of fluorescence labels can have largely overlapping emission spectra with only minute differences. Besides，for the optical coherent tomography(OCT)， a commonly used optical diagnosistechnology，wedevelopedvisible-lightOCT orvis-OCTto remedythelimitationthatthetraditionalOCTcannot usedforoxygenmetabolicimagingandweareapplyingvis-OCTtoinvestigateblindingdiseases.