The extract of Citri Sarcodactylis Fructus was used as raw material and treated with ⁶⁰Co-γ rays to enhance its polyphenols content. Its skin care effects, including moisturizing, skin irritation reduction, anti-oxidation, whitening, and anti-inflammatory properties, were evaluated. The results showed that the content of total polyphenols in the extract increased gradually with an increase in the adsorbed dose, and the scavenging efficiency of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals was positively correlated with the adsorbed dose. The extract of Citri Sarcodactylis Fructus has a high moisturizing rate under the conditions of color-changing silica gel and 46% humidity, and it is better than that of glycerin and sodium hyaluronate. The results of skin irritation analysis showed that skin exhibited a fair and smooth state after the phenomenon of cuticle peeling at high concentrations, and there was no subsequent skin irritation at low concentrations. In terms of antioxidation, the total phenolic content and DPPH radical scavenging efficiency of the extracts were positively correlated with adsorbed dose in the range of 0-50 kGy irradiation. It showed a good inhibitory effect on the production of melanin and tyrosinase in B16-F10 cells stimulated by α-MSH. After treatment with 30 kGy, the production of melanin in B16-F10 cells decreased by 48.97% and the activity of intracellular tyrosinase decreased to 42.41%. Additionally, the extract showed a strong inhibitory effect on the production and expression of inflammatory factors in LPS-treated RAW264.7 cells. The inhibition rate of IL-1β was 13.67% due to 30 kGy irradiation. The inhibition rates of IL-6 and TNF-α by 40 kGy irradiation were 27.01% and 6.88%, respectively. The skin care effect and safety of the irradiated extract of Citri Sarcodactylis Fructus were enhanced, making it highly suitable for practical applications.KEYEORDSCitri Sarcodactylis Fructus extract, Irradiation, Moisturizing, Irritating, Whitening, Anti-inflammatory