The purpose of this study is to investigate a relationship between the structure of lipid and colorectal carcinogenesis at the molecular level. Colorectal cancer specimens and adjacent normal tissues were measured using confocal Raman microscopy system. The bands at 2857 cm-1 and 2891 cm-1 originate from CH2 symmetric and asymmetric stretching vibrations,respectively. The band at 2938 cm-1 arises from symmetric C-H stretching vibrations. The results show that there are prominent differences between colorectal cancer specimens and adjacent normal tissues in Raman spectroscopy:1) The intensity of the bands of the cancer tissue spectrum at 2857 cm-1 and 2891 cm-1 are reduced compared with those of the normal spectra. The relative intensity of the band at 2938 cm-1 of lipid of colorectal cancer is stronger than that in normal tissues. 2) The average ratio (I2891 / I2857) values is reduced compared with those of the normal spectra. The values for normal and cancerous tissues are 2.21 and 1.69 by the function of ‘compare means’ of SPSS16.0 software. The research results demostrated that the lateral packing of lipids is loosened which may result in the fluidity of lipid to be increased and the interactions of lipid and protein increase when normal cells are transformed into cancerous cells. Our data suggested there is the relationship between the stretch vibrations of lipids and colorectal carcinogenesis.