To investigate the effect of the active ingredients of flavones in Ampelopsis grossedentata (dihydromyricetin and quercetin combined extract) on promoting the wound healing of photoelectric skin injury. Skin of rats and both fibroblasts and keratinocytes from human skin were injured by fractional carbon dioxide laser, respectively. The injured skin of rats and cells were treated by dihydromyricetin and quercetin after the laser injury, and then evaluated for skin wound healing and protein levels (interlukin-6 and tumor necrosis factor-α) of rats, reactive oxygen species fibroblasts, and cell migration of keratinocytes. Flavones in Ampelopsis grossedentata (dihydromyricetin and quercetin combined extract) decreased reactive oxygen species induced by laser injury in fibroblasts and proinflammatory factors (interlukin-6 and tumor necrosis factor-α) in skin, accelerated cell migration of keratinocytes, and promoted wound healing. Dihydromyricetin and quercetin combined extract promotes skin wound healing through inhibiting inflammation in laser-injured skin.