In recent years, trace physical evidence analysis techniques based on atomic absorption spectroscopy and plasma mass spectrometry have received increasing attention in modern forensic science. Compared with conventional analytical means, atomic absorption spectroscopy and plasma mass spectrometry techniques have the advantages of being fast, sensitive, and simple, but their sensitivity of these techniques is low. Therefore, there is an urgent need for an analytical instrument with high sensitivity, a large range, and the ability to analyze multiple trace elements simultaneously, to meet the requirements for rapid analysis of trace physical evidence in forensic science. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) is a method that can realize the simultaneous determination of major, trace, and ultra-trace elements. With the advantages of high sensitivity, low detection limit, high spatial resolution, easy operation, and simultaneous determination of multiple elements, this technique has been widely used in in situ microzonation analysis. This paper introduces the advantages of LA-ICP-MS in situ microzonation analysis. For the current status of the application of laser stripping inductively coupled plasma mass spectrometry in forensic science, it reviews the research progress of LA-ICP-MS in situ microzonation analysis technology in the analysis of physical evidence in forensic science, such as glass, hair, inks, gunshot residue (GSR), paper, soil, and so forth, and looks forward to the prospect of the application of this technology.