Experiments and simulations of multi-pulse laser damage CCD detectors were carried out. Using a laser with a wavelength of 1 064 nm and a pulse width of 100 μs, the laser damage experiment was performed on the CCD detector under the operating conditions of 1, 3 and 5 Hz. The three stages of multi-pulse damage CCD were observed and the CCD detection were obtained. The simulation model of multi-pulse laser damage CCD detector was established. The finite element method was used to simulate the damage threshold of CCD. The research shows that under the same frequency of laser irradiation, the damage threshold of CCD decreases with the increase of the number of laser pulses. When the number of pulses is constant, the repetition frequency is low, and the repetition frequency has little effect on the CCD damage threshold. The simulation results agree well with the experimental results.