In this work,a novel surface plasmon resonance (SPR)-based photonic crystal fiber (PCF) biosensor is investigated for real-time detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).By the interaction of the ligands on the sensing surface with the virus,the analyte′s refractive index (RI) ranges from 1.334—1.355.Three structures with different basic resonant materials are analyzed and evaluated based on the finite element method (FEM),including gold,silver,and silver with a titanium dioxide protective layer.The sensor′s wavelength works in visible,and the amplitude sensitivity (AS),wavelength sensitivity (WS),resolution,limit of detection (LoD) and figure of merit (FOM) reach up to -1 170.89 RIU-1,4 000 nm/RIU,2.5×10-5 RIU,6.3×10-9 RIU2/nm and 911.50 RIU-1,respectively.Results demonstrate that the proposed sensor has excellent potential in the detection of SARS-CoV-2,with excellent AS and LoD,which can be used for early and rapid detection.