In order to study the effect of laser processing parameters on the slit morphology and surface roughness of vascular stent, the experiment of optical fiber laser cutting of cardiovascular stent 316L was carried out by means of comparative analysis of different parameters. The effects of different process parameters such as laser pulse width, laser power, and cutting speed on the slit morphology and roughness of the material were analyzed, and the optimum combination of process parameters for laser cutting support was obtained. The results show that there are differences in slit morphology and surface roughness in different regions, in which the thickness of the vaporization zone is mainly affected by the pulse width and laser power. When the pulse width is 35μs, the maximum thickness of the slit vaporization zone can reach 120μm. In addition, the roughness of the slit vaporization zone of the support decreases at first and then increases with the increase of the cutting speed. When the cutting speed is 6mm/s, the lowest value of slit surface roughness is 650nm. The results lay a theoretical foundation for the research and subsequent finishing of cardiovascular stent optical fiber.