Aimed at eliminating the fluorescence interference from containers of the sample in the detection of Raman spectroscopy, a dual-axis confocal detection scheme is proposed based on the characteristic that the fluorescence generated by the container and the Raman signal of the sample are not confocal. The experimental results show that the fluorescence interference signals from the container are reduced by an order of magnitude,and the intensity of Raman signal decreases by about 30%. The dual-axis confocal Raman detection scheme can solve the mixed reception problem of Raman signal and fluorescence in the conventional coaxial confocal Raman detection scheme, solve the limitation of the dynamic detection range of Raman signal caused by the fluorescence interference, and achieve the effective detection of Raman signal of the material in fluorescent material containers.