The graft copolymer (CPGMA) made from bleached chemical eucalyptus pulp (BCEP) and glycidyl methacrylate (GMA) was reacted with ethylenediamine (EDA) to synthesize the polyamine-type cellulose-based chelating fiber CPGMA-EDA. The chelating fiber was characterized by means of Fourier transform infrared spectroscopy (FTIR), 13C cross polarization magic angle spinning NMR spectra (13C CP/MAS NMR) and X-ray diffraction (XRD). The results of FTIR and 13C CP/MAS NMR show that the characteristic absorption peak (904 cm-1) of epoxy group in CPGMA disappeared, and the absorption peak around 3 200-3 500 cm-1 broadened due to the vibration of new formed N—H bond while the CPGMA reacted with EDA. Both of these indicate that amino group was grafted onto CPGMA through the ring-opening reaction. The XRD results indicate that the crystallinity of CPGMA-EDA was 48.1%, and decreased by 31.1% compared to that of BECP, indicating that the reaction not only takes place in the noncrystal regions, but also in the crystal regions of cellulose.