Copper-base antibacterial glass with high strength ，good transparency ，and excellent antibacterial effect was prepared by dipping 0.55mm flat lithium aluminum silicate glass in a mixture of KNO3 /NaNO3 molten salt as the first step of chemical tempering（IOX1）and KNO3 /NaNO3 /CuSO4 mixed molten salt as the second step of chemical tempering（IOX2）. The effect of different ratio of CuSO4 on the antibacterial properties of the glass was investigated. The results showed that the first step was to exchange in the mixed salt with mass ratio w（KNO3）∶ w（NaNO3）was 50∶50 by soaking for 4 hours.In the second step，the mass ratio of mixed salt for ion exchange w（KNO3）∶ w（NaNO3）∶ w（CuSO4）was 90 ∶5 ∶5，and the soaking time was 2.5 hours.Under this process condition ，the excellent antibacterial glass with a surface stress value more than 700 MPa ，a transmittance of over 90% in the visible light region and an antibacterial activity value R ≥ 5 can be obtained.