The FeCrNiCoMn high-entropy alloy coating with nice surface topography is prepared on 45# steel by laser cladding. In order to study the property of resistance to high temperature softening of the coating, the annealing experiments of coating are performed at 550 ℃, 700 ℃, 900 ℃, 1000 ℃, 1160 ℃ for 2 h, respectively. The microstructure, phase structure and microhardness of the coatings annealed at different temperatures are investigated by scanning electron microscope (SEM), X-ray diffraction (XRD) and microhardness tester, respectively. The results show that the coating after laser cladding is mainly composed of typical dendrites and exhibits simple face-centered cubic (FCC) and minor body-centered cubic (BCC) structure phases with average microhardness of 540HV0.2. The microstructure of the coating grows up slightly after being annealed at 550 ℃, 700 ℃ and 900 ℃. However, the coating forms relatively more BCC phase when anneals at 900 ℃. While at 1000 ℃ and 1160 ℃, the microstructure grows up gradually, and the phase structure transforms into single FCC structure. As the annealing temperature increases, the microhardness of the FeCrNiCoMn cladded coating increases firstly, and then decreases, the highest microhardness is 665HV0.2 after being annealed at 900 ℃. It indicates that the FeCrNiCoMn high-entropy coating after laser cladding exhibits nice tempering resistance below 900 ℃.