Lipid transfer proteins （LTPs） are a class of small molecular transport proteins widely present in plant cells, primarily responsible for the transport of lipids and the delivery of various biochemical reaction products. In this study, based on the previously identified drought stress-responsive gene SiLTP1 in millet and the obtained positive transgenic millet lines overexpressing SiLTP1, lipidomic analysis under salt stress was performed．GC-MS analysis revealed that the levels of digalactosyldiacylglycerol (DGDG) and monogalactosyldiacylglycerol（MGDG） were the highest in the seedlings of millet variety Ci846 and the SiLTP1-overexpressing lines. After treatment with 200 mmol·L^-1 NaCl, the levels of DGDG/MGDG in the leaves of SiLTP1-overexpressing millet seedlings were higher than those in Ci846. At the same time, the levels of phosphatidylglycerol （PG） and diglycerides （DG） also increased. Based on these results, it is suggested that lipid transfer proteins can modulate the content of phospholipids and lipid molecules in the SiLTP1-overexpressing millet seedlings, thereby participating in the response to salt stress in millet. This study provides theoretical support and candidate gene resources for the systematic analysis of the mechanisms through which lipid transfer proteins participate in millet's salt stress response，offering a foundation for the development of salt-tolerant millet varieties.