A scheme that utilizes the 4-level pulse-amplitude-modulated (4PAM) signal as the re-modulated signal of colorless optical network units (ONUs) based on reflection semiconductor optical amplifiers (RSOAs) is proposed. The system with 10-Gb/s non-return-to-zero (NRZ) downstream and 5-Gb/s 4PAM upstream signals is theoretically analyzed and experimentally verified. Simulation and experimental results reveal that the 4PAM re-modulated signal yields better performance than the NRZ signal in the upstream. A receiver power penalty of 1.6 dB is also improved by the 4PAM signal at back-to-back (BtB) transmission, whereas another receiver power penalty of 1.5 dB improved after 30-km single mode fiber transmission, where 4PAM signals are used as upstream signal.