Fulgide, a kind of thermally irreversible photochromic compound, can be used for polarization holographic recording owing to its photoinduced anisotropy and photochromic property under the irradiation of linear polarization light. In this letter, a new technique of optical image operation based on the polarization multiplexing scheme in the fulgide film is demonstrated, which can implement the readout of two individual orthogonal polarized images separately and the subtraction or summation of the two images by simply rotating a polarizer in front of the charge-coupled device (CCD) detector.