In order to improve the trapping efficiency of the double-tapered optical fiber probe (DOFP), the first taper angle was fabricated by the static chemical etching method, and then the second taper angle was fabricated by the polishing method. Trapping experiment of a yeast cell was completed using a single optical fiber tweezer system based on DOFP. In addition, a DOFP with similar parameters was fabricated by interfacial layer etching method. The trapping force of two DOFPs fabricated by two different methods on the yeast cell was measured. The results show that, DOFP fabricated by the hybrid method has better surface smoothness and symmetry. Its trapping efficiency is 20% higher than that of DOFP fabricated by the interfacial layer etching method. This study is helpful for the fabrication of double-tapered optical fiber probes using the hybrid method.